I'm wondering anyone of you here have been knowing the different between sepharose high performance and sepharose Fast flow? What is the different between this two? Are they different in term of the binding efficiency, flow rate, appication or resin type? and what is the difference of these two with capto?
For Laboratory and E.coli system you can use FF. You have a better velocity for HP when you load big volume what yeast system (liters of supernatant). For capto, the groups are linked to a high flow agarose base matrix modified with a dextran surface extender which further increases capacities and mass transfer properties. (you use less bed height). The resin choice, it's a process development for scaling up.
This handbook will provide useful information for you.
https://cdn.cytivalifesciences.com/api/public/content/digi-11639-pdf?_gl=1*dae9sc*_gcl_au*MTI3MzE4NDA0OC4xNzI1OTI3NTE2
Nicolas Poirier I'd like to thank you for sharing this information.
As I found, i can say that capto resins significantly better flow rate performance and higher productivity than F.F and H.P.
yes capto, it can be a good choice as a first step of purification.
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