Homasadat Esfahani

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Questions related from Homasadat Esfahani

Why is trifluoroacetic acid (TFA) used in c-18 column?

I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.

24 July 2024 8,056 3 View

What are the best alternatives for PMSF in preservation of protein in extraction human papillomavirus protein from Saccharomyces cerevisiae?

I wish to protect the protein from proteinase enzymes when using (yeast) to purify human papillomavirus protein, Which enzyme inhibitor is alternative to PMSF? which to be stable in high...

25 July 2023 7,258 0 View

Is it possible in cell cycle arrest we see sub-G1and G2 arrest at the same time?

To determine whether changes in cell cycle are related to DNA damaged, we investigated the effect of our drug on cell cycle distribution by flow cytometric analysis. also the tested drug,...

19 August 2021 4,041 1 View

Can antagonism results of two drugs change to synergism in higher dose?

I calculated the combination index with CompuSyn software, Among 8 combination data points, 5 of them are on the antagonism side (CI>1), the other 2 points are nearly additive and one of them is...

21 July 2021 739 7 View