Homasadat Esfahani

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Questions related from Homasadat Esfahani

Inquiry Regarding Nickel Sepharose Resin Color Change and CIP Protocols?

Dear Colleagues, I am encountering an issue with my Nickel Sepharose resin, as it becomes colored after a run. I would like to seek your advice on potential solutions to this problem and would...

16 December 2024 8,023 1 View

While cysteine has a positive hydropathy index, why is it categorized as a polar amino acid?

Cysteine has a hydropathy score that reflects its overall preference for non-polar environments compared to some other polar amino acids, meaning it can be somewhat hydrophobic. Its positive...

16 November 2024 9,601 2 View

Why do I see degradation of my target proteins during purification using Nickel resins?

Hi everyone, I'm working with a soluble recombinant protein that contains a SUMO tag, which facilitates the downstream purification process. However, when I tried to purify the protein using...

12 November 2024 2,933 6 View

How to purify glycoprotein by chromatography? which resin do you recommended?

I try to purify a glycosylated protein that is secreted and glycosylated (expressed without histag) using Pichia pastoris. I used different ion exchange chromatography while 90% of glycosylated...

08 October 2024 7,804 7 View

What is the different between sepharose high performance and sepharose Fast flow?

I'm wondering anyone of you here have been knowing the different between sepharose high performance and sepharose Fast flow? What is the different between this two? Are they different in term of...

09 September 2024 6,156 5 View

Why is trifluoroacetic acid (TFA) used in c-18 column?

I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.

24 July 2024 8,351 3 View

What are the best alternatives for PMSF in preservation of protein in extraction human papillomavirus protein from Saccharomyces cerevisiae?

I wish to protect the protein from proteinase enzymes when using (yeast) to purify human papillomavirus protein, Which enzyme inhibitor is alternative to PMSF? which to be stable in high...

25 July 2023 7,321 0 View

Is it possible in cell cycle arrest we see sub-G1and G2 arrest at the same time?

To determine whether changes in cell cycle are related to DNA damaged, we investigated the effect of our drug on cell cycle distribution by flow cytometric analysis. also the tested drug,...

19 August 2021 4,101 1 View

Can antagonism results of two drugs change to synergism in higher dose?

I calculated the combination index with CompuSyn software, Among 8 combination data points, 5 of them are on the antagonism side (CI>1), the other 2 points are nearly additive and one of them is...

21 July 2021 800 7 View