Better have paraffin embedded and decalcinatef tissues. It gives you opportunity to use this tissue in future. For example for immunohistochemistry, ISH or just additional histochemical stains

I have always used paraffin-embedded sections of knee joints. May be cryo section means, after decalcification, you can freeze tissue in O.C.T. in liquid nitrogen and do cryosectioning using a cryostat. That saves you lot of steps. In any case, you need to do decalcification of bony tissue.

Better have paraffin embedded and decalcinatef tissues. It gives you opportunity to use this tissue in future. For example for immunohistochemistry, ISH or just additional histochemical stains

Dear Dongyeon Nam: Respectfully, you will be losing a monumental amount of information by removing the mineral. The adaptation, maintenance, and repair of bone tissue, particularly diseased tissue, concerns the association between the organic and inorganic phases, and to remove 70% of the tissue by weight as mineral will be to throw away all that this phase can tell you about its history. A plastic embedded histological ground thin section of the tissue will provide all of the transmitted light LM, PLM, DIC (etc.) potential, which, together with the appropriate surface stains, can provide mineralization density variability of interest (if you do not have BSE-SEM). In addition, while not all immunohistochemistry stains have been experimented with, the usual ones that one would use for arthritic bone (e.g., TRAP) do work. Consider performing your work on the intact tissue, not decalcified.

Hi Dongyeon!

I will recommend to do paraffin embedded sectioning for your tissue. Altough you can try for OCT embedded sectioning, its quite difficult to get good quality section from bones. I did frozen sectioning only when i had to use tissue from reporter mice.

Paraffin embedded tissue slides are easier to store for long time; possible to perform most of the staining including IHC and get good quality images. These are few advantages of paraffin over frozen sectioning.

Altough tissue processing steps needed before any experiment (like staining) are more with paraffin than frozen tissue, still i think paraffin sectioning is better specifically for your tissue.

Hope this will help you to find differences.