Hello Eman Gaballah
I would suggest that a more physiological relevant approach would be to use anti-CD3/CD28 to stimulate T cells in a manner that partially mimics stimulation by antigen presenting cells.
Anti-CD3 plays a central role in T cell proliferation. Anti-CD3 delivers a strong proliferative signal through the T cell receptor complex, but in the absence of additional costimulatory signals the resulting proliferation is often followed by premature T cell apoptosis or anergy. So, by using anti-CD3/CD28 to simultaneously deliver both anti-CD3 and the costimulatory anti-CD28 signals, proliferation can be increased without provoking early cell death. Moreover, the expanding cells will also show enhanced ability to release cytokines and lyse targets cells. So, I would recommend using anti-CD3/CD28 in culture.
I hope this helps.
Best.
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