What is the difference between wound healing and migration and what is there significance in hepG2 cells. What kind of culture medium (serum free or medium with serum) can be used for these assays?
In wound healing you have a confluent monolayer that you then remove partially by a pipette tip, chemical agent, by cryofreezing and the like. Then you check after a certain timepoint (eg. 24h) how much of the wounded area is covered again. Therefore, in wound healing you will usually have proliferation, migration and cell spreading (increasing cell size). Thus, in wound healing assay you can check whether a chemical agent has an influence on any of those properties. Using serum free medium will reduce the effect of proliferation and is often used when you have fast proliferating cells.
In a migration assay, you usually check how many cells can go through a certain medium (gel or porous membrane). Therefore, you do not check for cell spreading and depending on the timepoint, also proliferation plays a minor role. Migration assays are used to assess the chemoattractive properties of a chemical agent or the effect on metastatic behavior of cancer cells
Parts of response can be find in the attached documents.
I have not too much experience with HepG2 cells. I thus prefer that RG experts will provide you with sound responses.
Best regards
Robert
Thank you very much for looking at my question Robert I am thankful to you for your references and I really appreciate your effort.
In wound healing you have a confluent monolayer that you then remove partially by a pipette tip, chemical agent, by cryofreezing and the like. Then you check after a certain timepoint (eg. 24h) how much of the wounded area is covered again. Therefore, in wound healing you will usually have proliferation, migration and cell spreading (increasing cell size). Thus, in wound healing assay you can check whether a chemical agent has an influence on any of those properties. Using serum free medium will reduce the effect of proliferation and is often used when you have fast proliferating cells.
In a migration assay, you usually check how many cells can go through a certain medium (gel or porous membrane). Therefore, you do not check for cell spreading and depending on the timepoint, also proliferation plays a minor role. Migration assays are used to assess the chemoattractive properties of a chemical agent or the effect on metastatic behavior of cancer cells
Dear Chand,
Here attached are two films that illustrate the comments of Andreas that I fully share.
I am also attaching an article illustrating the notion of chemotaxis.
Best regards
Robert
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