I've heard that some viruses can only infect E6, and I have no idea why. Anyone
I am expressing furin gene in a furin deficient cell line (LoVo), but I always get 2 bands on my western blot ~ 87kDa, corresponding to furin expression and a lower band, of approximately 50kDa,...
08 September 2018 1,784 0 View
I am having some problems trying to clone a mRNA, maybe due to it's amount compared to other mRNA. I have tried PCR using cDNA from oligo DT, specific or random primers, but with no success. I was...
07 August 2018 3,521 3 View
Does anyone work with expression in S2 cells? How efficient is the transfection using Lipofectamine 2000? Everytime I try to transfect using CaCl2 I get a cloudy media, and it seems that the...
06 July 2018 1,480 0 View
I am trying to establish a stable cell line after transfection with pcoHygro plasmid. Every time I supplement with the selection antibiotic (hygromycin) I get some small contamination (it seems...
06 July 2018 9,814 6 View
Is it possible to perform a PCR, using as the reverse primer a oligo dT, the same used in the reverse trancription? I am trying to amplify a mRNA, but with no success
05 June 2018 6,864 4 View
I need to produce antibodies against my protein in 2 different animals, amd my university provide us mice and rats, but I am not sure if the secondary antibody will cross react between those...
01 February 2018 812 3 View
I am expressing a protein using Baculovirus / SF9 cells system, and I was wondering if, with the signal peptide for exporting the protein (pAcGP67a plasmid), the protein will go through Golgi...
09 October 2017 6,387 0 View
The reads are not assembled as contigs, and I have tried some different softwares with no success, such as EPI2ME and Geneious. I have no idea how to align and check my sequences, I have tried...
08 September 2017 2,998 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View