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Questions related from Danillo Esposito
I am expressing furin gene in a furin deficient cell line (LoVo), but I always get 2 bands on my western blot ~ 87kDa, corresponding to furin expression and a lower band, of approximately 50kDa,...
09 September 2018 1,769 0 View
I am having some problems trying to clone a mRNA, maybe due to it's amount compared to other mRNA. I have tried PCR using cDNA from oligo DT, specific or random primers, but with no success. I was...
08 August 2018 3,508 3 View
Does anyone work with expression in S2 cells? How efficient is the transfection using Lipofectamine 2000? Everytime I try to transfect using CaCl2 I get a cloudy media, and it seems that the...
07 July 2018 1,462 0 View
I am trying to establish a stable cell line after transfection with pcoHygro plasmid. Every time I supplement with the selection antibiotic (hygromycin) I get some small contamination (it seems...
07 July 2018 9,802 6 View
Is it possible to perform a PCR, using as the reverse primer a oligo dT, the same used in the reverse trancription? I am trying to amplify a mRNA, but with no success
06 June 2018 6,848 4 View
I need to produce antibodies against my protein in 2 different animals, amd my university provide us mice and rats, but I am not sure if the secondary antibody will cross react between those...
02 February 2018 795 3 View
I am expressing a protein using Baculovirus / SF9 cells system, and I was wondering if, with the signal peptide for exporting the protein (pAcGP67a plasmid), the protein will go through Golgi...
10 October 2017 6,374 0 View
The reads are not assembled as contigs, and I have tried some different softwares with no success, such as EPI2ME and Geneious. I have no idea how to align and check my sequences, I have tried...
09 September 2017 2,981 3 View
I've heard that some viruses can only infect E6, and I have no idea why. Anyone
02 February 2016 1,025 0 View
