I am currently working on both Vero and MA104 cell lines. Both cell lines have been procured from ATCC and as per the technical document, both cell lines have similar source (origin) animal yet MA104 cells show a higher sensitivity to enteric virus infections.
I analyzed the cell DNA using multiple sequence alignment only to find that they are 99% identical in their genome (as per data from published sequences of ATCC).
I would like to know if there are any cell surface proteins which enable the MA104 cells to demonstrate higher sensitivity to enteric virus (e.g. rotavirus) infections as compared to Vero cells.
Hello! I had to deal with the properties of VERO cell culture. And now I also work with MA104 cells. The fundamental difference between Vero cell culture and MA104 cell culture is their defectiveness in the production of interferon. See a list of related old publications:
Emeny J.M., Morgan M.J. Regulation of the interferon system: evidence that Vero cells have a genetic defect in interferon production // J. gen. Virol. – 1979. – V.43. – P. 247-252. DOI:10.1099/0022-1317-43-1-247.
Diaz M. O., Ziemin S., Le Beau M. M. et al. Homozygous deletion of the alpha- and beta 1-interferon genes in human leukemia and derived cell lines // Proc. Natl. Acad. Sci. USA. – 1988. – Vol. 85. – P. 5259–5263.
Desmyter J., Melnick J. L., Rawls W. E. Defectiveness of interferon production and of rubella virus interference in a line of african Green Monkey kidney cells (Vero) // Journal of Virology. – 1968. – Vol. 2, №10. – P. 955–961. PMID 4302013.
Osada N, Kohara A, Yamaji T, et al. (2014). The genome landscape of the African green monkey kidney-derived Vero cell line // DNA Research. – 2014. – Vol. 21, №6. – P. 673–83. DOI:10.1093/dnares/dsu029.
Kim W.Y., Sharpless N.E. The regulation of INK4/ARF in cancer and aging // Cell. – 2006. – Vol.127. – P. 265-275.
Yang L., Luquette L.J., Gehlenborg N. et al. Diverse mechanisms of somatic structural variations in human cancer genomes // Cell. – 2013. – Vol. 153. – P. 919-929.
Ohara H. Cytogenetic examination of Vero cells derived from the present stock. In: Simizu B., Terasima T., editors. Vero cells: origin, properties and biomedical applications. / Chiba, Japan: Department of Microbiology School of Medicine Chiba University. – 1988. – P. 36–38.
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