Please how can I justify my regression results?

I conducted a study to investigate stock market reaction to equity issues announcement and i ended up with 64 announcements and i then used the announcement day returns as my DV and investigated...

14 January 2021 1,127 3 View

Can anyone recommend a laboratory virtual simulator (or similar approach: videos, etc.) , to teach genetics laboratory pratices online?

I am interested in any resources that would help with a genetics laboratory course online. I know it is going to be difficult... but this is how things are for the moment, and we need to adapt....

20 September 2020 2,678 3 View

Is it dangerous for me to handle Ethyl methanesulfonate (EMS) in an open air space?

18 June 2020 3,918 3 View

How to correctly indicate a zinc finger in a protein for molecular dynamics?

I am preparing a protein that has a Zn finger for molecular dynamics. To indicate the finger of Zn a patch is placed in which the deprotonation of the cysteines is necessary so that the Zn ion can...

05 March 2020 1,425 3 View

No title

28 February 2020 1,633 2 View

Looking for a manual about NIRS in cognitive science / neuropsychology, suggestions?

Dear community, i'm looking for a manual about NIRS, functioning, methodology and data analysis. in the field of cognitive science / neuropsychology. I would like to know as much as possible...

30 January 2020 8,861 2 View

How do I calculate solar absorbance and thermal emittance of absorber coating from reflectance spectrum using standard AM 1.5 and blackbody radiation?

I have a reflectance data of my solar absorber coating from UV-Vis, I want to calculate the solar absorbance of my coating using standard reference spectrum AM 1.5 and thermal emittance of thesame...

15 November 2019 225 3 View

How can Iimprove the resolution on a GC?

When I run a O2 and N2 standard in the gas chromatograph, the peaks are overlapped, How can I improve the resolution of the chromatogram. This situation is new, last monththe calibrationwent just...

22 August 2019 4,050 6 View

On a EVO LS10 SEM, I can see images on spot 0 of the aperture, however on spot 1 and 2, I cant see any images. what could be responsible for this?

I an using the EVO LS10 SEM from Zeiss, I can see images on spot 0 of the aperture, however when the aperture is switched to spot 1 or 2, I am unable to see any image. Please what could be...

05 February 2019 3,902 1 View

How is witness coaching regulated in your Country?

I am doing a research about ethics in international arbitration so I need to know how witness coaching is dealt with in various Countries. Thank you in advance for your cooperation!

03 February 2019 7,212 9 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View