11 Questions 6 Answers 0 Followers
Questions related from Bernardo Bello Ortí
I would like to know if proteins expressed in higher quantities, such as DNA polymerase, would be better vaccine candidates for a T-cell based vaccine. Thanks, Bernardo
24 October 2014 9,230 6 View
I'm looking for tools to semi-automate finding non-coding RNAs in bacterial RNA-seq data. I've been able to find some tools but I'm still quite lost (see link below). I've also seen this Biostar...
31 March 2014 5,339 2 View
I've been able to perform GO enrichment but since 40% of the bacterial genome I'm working has no GO term assigned, its possible I'm loosing important results. I'm looking for alternatives: I've...
25 March 2014 1,698 1 View
We found a 200 fold change regulation (with good read coverages). Has anyone observed this?
04 March 2014 7,753 5 View
I would like to demonstrate that Haemophilus influenzae, a common human pathogen, lacks numerous genes in comparison with its ancestor due to genome reduction. I need to know its ancestor to...
25 February 2014 4,825 2 View
I would like to perform whole genome identification of pseudogenes. Could anyone suggest a good strategy?
16 February 2014 4,290 2 View
What's the difference between these two filtering alternatives when looking for all proteins available for a selected organism in NCBI? I get the double of proteins in 'noexp' case!
06 February 2014 9,171 2 View
I would like to know your opinions about the correlation I obtained between qPCR and RNA-seq for 8 genes, two of them being reference genes. The r2 is good but I worry that qPCR FC are not as high...
25 January 2014 7,362 16 View
Hi, I would like to know the cDNA quantity you recommend for the mentioned PCR. It will be used to validate RNA-Seq results. Thanks, Bernardo
13 January 2014 2,849 11 View
Basically, I'm looking for the same table than this one (http://genomesonline.org/documents/Export/gold.xls) and this one from GOLD genomes (http://genomesonline.org/cgi-bin/GOLD/index.cgi), but...
16 December 2013 3,288 4 View
I've seen in MIQE guidelines that calibration curves a needed when performing RT-qPCR. I'll use genomic DNA to perform these calibration curves. My question is that I don't know the range of...
04 December 2013 289 3 View