Negative charge nanoparticles cellular internalization versus positive ones

is negatively charged NPs preffered as vector for DNA than cationic one is it targeting to liver

04 May 2016 6,434 2 View

What steps should I take if my tissue sample for RT PCR becomes contaminated with formaline?

I take liver samples from rats to perform RNA extraction and real time PCR but these livers immersed in formaline for one hour i wash it with saline so i want to ask what is the effect of...

06 July 2015 1,700 5 View

How can I stop the aggregation of zein nanoparticles in treatment medium after treatment to cells?

04 May 2015 2,108 3 View

How can I overcome nanoparticle aggregation in dmem tissue culture mediation ?

04 May 2015 8,596 2 View

Why did I get A260/A280 above two after alkali lysis method for DNA plasmid extraction?

10 November 2014 9,198 7 View

Why is the Hoechst 33258 dye for DNA quantification using jasco fluorometer giving me no stable reading but goes up and down?

The reading is not stable, and the reading is moving up and down and is never stable. Please advise.

09 October 2014 312 2 View

How can I use ethidium bromide to measure DNA concentration fluorimetry?

I want a protocol to measure DNA concentration fluorimetry using ethidium bromide dye.

05 June 2014 1,967 12 View

Can I use vancomycin rather than kanamycin for bacteria contain pEGFP-C3?

or any antibiotic rather than Kanamycin as it's not available

01 February 2014 4,074 3 View

What is an average yield of PCR product for human gene?

PCR product size 1.2 kb optimum yield after 30 cycle 20 ul reaction

31 December 2013 3,583 3 View

How many PCR runs are sufficient to create 1 mg your target DNA fragment?

I need 1mg DNA sample could be synthesized by how many PCR runs?

10 November 2013 9,345 13 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View