Negative charge nanoparticles cellular internalization versus positive ones

is negatively charged NPs preffered as vector for DNA than cationic one is it targeting to liver

04 May 2016 6,434 2 View

What steps should I take if my tissue sample for RT PCR becomes contaminated with formaline?

I take liver samples from rats to perform RNA extraction and real time PCR but these livers immersed in formaline for one hour i wash it with saline so i want to ask what is the effect of...

06 July 2015 1,700 5 View

How can I stop the aggregation of zein nanoparticles in treatment medium after treatment to cells?

04 May 2015 2,108 3 View

How can I overcome nanoparticle aggregation in dmem tissue culture mediation ?

04 May 2015 8,596 2 View

Why did I get A260/A280 above two after alkali lysis method for DNA plasmid extraction?

10 November 2014 9,198 7 View

How can I use ethidium bromide to measure DNA concentration fluorimetry?

I want a protocol to measure DNA concentration fluorimetry using ethidium bromide dye.

05 June 2014 1,967 12 View

Can I use vancomycin rather than kanamycin for bacteria contain pEGFP-C3?

or any antibiotic rather than Kanamycin as it's not available

01 February 2014 4,074 3 View

What is the difference between phenol:chloroform:IAA of pH 6.7 and pH 8?

There are 2 phenol: choloroform: isoamylalcohol on market one has pH 6.7 and the other a pH of 8. What's difference between them and which is better for DNA extraction?

01 February 2014 4,853 3 View

What is an average yield of PCR product for human gene?

PCR product size 1.2 kb optimum yield after 30 cycle 20 ul reaction

31 December 2013 3,583 3 View

How many PCR runs are sufficient to create 1 mg your target DNA fragment?

I need 1mg DNA sample could be synthesized by how many PCR runs?

10 November 2013 9,345 13 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View