Shaimaa Hassan

15 Questions 21 Answers 0 Followers

Questions related from Shaimaa Hassan

Negative charge nanoparticles cellular internalization versus positive ones

is negatively charged NPs preffered as vector for DNA than cationic one is it targeting to liver

05 May 2016 6,468 2 View

What steps should I take if my tissue sample for RT PCR becomes contaminated with formaline?

I take liver samples from rats to perform RNA extraction and real time PCR but these livers immersed in formaline for one hour i wash it with saline so i want to ask what is the effect of...

07 July 2015 1,735 5 View

How can I overcome nanoparticle aggregation in dmem tissue culture mediation ?

my nanoparticle is aggregated in dmem during tissue culture how can i overcome this problem?if i can incubate my nano with cells for certain time till uptaking some of my nano without media then...

05 May 2015 8,634 2 View

How can I stop the aggregation of zein nanoparticles in treatment medium after treatment to cells?

zein nanoparticle tend to aggregate and coagglutinate when i added it to DMEM media to treat cellsI use tween80 as surfactant \ pluronicF86.can I incubate the cells with nanoparticle dissolved in...

05 May 2015 2,146 3 View

Why did I get A260/A280 above two after alkali lysis method for DNA plasmid extraction?

I did alkali lysis of my bacteria and I got DNA concentration of 4000ng/µL, A260/A280 = 2.2 and A260/A230 of about 1.9 - what would be the expected contaminants and what shall I do?

11 November 2014 9,233 7 View

Why is the Hoechst 33258 dye for DNA quantification using jasco fluorometer giving me no stable reading but goes up and down?

The reading is not stable, and the reading is moving up and down and is never stable. Please advise.

10 October 2014 347 2 View

How can I use ethidium bromide to measure DNA concentration fluorimetry?

I want a protocol to measure DNA concentration fluorimetry using ethidium bromide dye.

06 June 2014 2,026 12 View

Can I use vancomycin rather than kanamycin for bacteria contain pEGFP-C3?

or any antibiotic rather than Kanamycin as it's not available

02 February 2014 4,114 3 View

What is the difference between phenol:chloroform:IAA of pH 6.7 and pH 8?

There are 2 phenol: choloroform: isoamylalcohol on market one has pH 6.7 and the other a pH of 8. What's difference between them and which is better for DNA extraction?

02 February 2014 4,894 3 View

What is an average yield of PCR product for human gene?

PCR product size 1.2 kb optimum yield after 30 cycle 20 ul reaction

01 January 2014 3,624 3 View

How to measure the concentration of a small amount of DNA to 0.02ng/microlitre?

I am in need of an inexpensive, reproducible and sensitive method. Could I use ethidium bromide?

11 November 2013 8,520 7 View

What is the protocol of phenol:chloroform:IAA preparation?

I need protocol for adjusting ph using which buffer.

11 November 2013 5,565 18 View

How many PCR runs are sufficient to create 1 mg your target DNA fragment?

I need 1mg DNA sample could be synthesized by how many PCR runs?

11 November 2013 9,378 13 View

How does one calculate the required amount of primers required for PCR reaction?

How does one calculate the required primer concentration to be added to PCR Mastermix?

11 November 2013 4,867 10 View

Can I use nanodrop to measure DNA concentration after PCR run?

What is the blank used in this case? I want to know the concentration of DNA after PCR run. Can I simply use nanodrop or other component of mastermix would interfere with my result?

11 November 2013 6,747 27 View