What is the difference between PCR-tetra primer ARMS, PCR-AS, and PCR-CTPP?

More Noha Said's questions See All

HOW can i minimize the number of hits made by the designed primer to only the intended sequence?

i have designed a primer but i found it gives 200 hits over than the intended sequence (5 of them in the organism that i work with). this give poor pcr results. i want to avoid this but i don,t...

08 September 2018 7,012 3 View

Could any one help me in designing accurate primers for the following sequence?

i had designed primers for two sequences using blast tools in NCBI but they gave poor amplification lower than the ladder after gradient pcr i want any one to check them and help me in designing...

08 September 2018 7,948 4 View

How to calculate delta CT for calibrator in qpcr in case-control study ?

i do a study for a gene expression in HCC patients compared to healthy person using real time pcr. i have analyzed ct for gene of interest and house keeping gene in each sample either HCC or...

06 July 2018 7,002 13 View

What is the best PCR condition (PCR mix and cycles and temperature ) for amplification of a WNT10A?

I have used the following procedures but am still having strong primer dimer bands while the desired product is absent. The cycling conditions for PCR program that i used were 5 min at 95C for...

02 March 2018 6,091 6 View

Is it useful for doing conventional PCR before REAL TIME PCR to be sure of the product size and primer annealing?

i will do a real time PCR for miRNA after revrse transcription. iam afraid from bad results and wasting mu kits which is expensive. i want to know whether it is possible to do conventional pcr and...

02 March 2018 3,934 12 View

How can i make pedigree file for using in haploview software ?

i want to make haplotype and linkage analysis. i found that the most common programme is the haploview. but i don,t know how to make pedigree or linkage or premakeped file to use in the haploview....

02 March 2016 1,050 0 View

What is the difference betwwen gold and green open access?

i want to choose the green open access because it is free but i want to know every thing about it

01 February 2016 3,191 4 View

Is it better ti use triazole or total RNA or miRNA isolation kit to isolate miRNA (less than 200 nt) from serum or plasma?

i want to know which method is suitable to extract miRNA from serum or plasma use triazole or total RNA column spin or miRNA column spin isolation kit . many studies used the first first and the...

10 November 2015 7,389 8 View

Do we use total RNA extraction kit or mi RNA extraction kit to extract mi RNA from serum?

What is better, the use of total RNA extraction kit or mi RNA extraction kit to extract miRNA from serum or plasma . What is better for whole blood?

10 November 2015 5,922 7 View

Is qRT-PCR one step or two step better for miRNA?

i want to know what is better for miRNA THE ONE STEP OR TWO STEP PROTOCOL ( ACCURACY AND COST)

10 November 2015 3,328 3 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Arun Prabhu Dhanapal

All three are used for SNP genotyping/ allele calling. You can look at the description of each in following literature's and protocol to use them are also in same literatures. Good Luck.

Noha Said

THANK YOU

Iman Hassan Ibrahim

PCR-CTPP worked perfectly with me, except in primer design which is not as easy as primer design for traditional PCR. You just have to keep an eye on reaction conditions esp. Tm of the primers and annealing temperature .