HOW can i minimize the number of hits made by the designed primer to only the intended sequence?

i have designed a primer but i found it gives 200 hits over than the intended sequence (5 of them in the organism that i work with). this give poor pcr results. i want to avoid this but i don,t...

08 September 2018 6,894 3 View

Could any one help me in designing accurate primers for the following sequence?

i had designed primers for two sequences using blast tools in NCBI but they gave poor amplification lower than the ladder after gradient pcr i want any one to check them and help me in designing...

08 September 2018 7,829 4 View

How to calculate delta CT for calibrator in qpcr in case-control study ?

i do a study for a gene expression in HCC patients compared to healthy person using real time pcr. i have analyzed ct for gene of interest and house keeping gene in each sample either HCC or...

06 July 2018 6,703 13 View

Is it useful for doing conventional PCR before REAL TIME PCR to be sure of the product size and primer annealing?

i will do a real time PCR for miRNA after revrse transcription. iam afraid from bad results and wasting mu kits which is expensive. i want to know whether it is possible to do conventional pcr and...

02 March 2018 3,804 12 View

What is the best PCR condition (PCR mix and cycles and temperature ) for amplification of a WNT10A?

I have used the following procedures but am still having strong primer dimer bands while the desired product is absent. The cycling conditions for PCR program that i used were 5 min at 95C for...

02 March 2018 5,925 6 View

How can i make pedigree file for using in haploview software ?

i want to make haplotype and linkage analysis. i found that the most common programme is the haploview. but i don,t know how to make pedigree or linkage or premakeped file to use in the haploview....

02 March 2016 917 0 View

What is the difference betwwen gold and green open access?

i want to choose the green open access because it is free but i want to know every thing about it

01 February 2016 3,075 4 View

Is it better ti use triazole or total RNA or miRNA isolation kit to isolate miRNA (less than 200 nt) from serum or plasma?

10 November 2015 7,260 8 View

Is qRT-PCR one step or two step better for miRNA?

10 November 2015 3,207 3 View

Do we use total RNA extraction kit or mi RNA extraction kit to extract mi RNA from serum?

10 November 2015 5,794 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View