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Questions related from Noha Said
i have designed a primer but i found it gives 200 hits over than the intended sequence (5 of them in the organism that i work with). this give poor pcr results. i want to avoid this but i don,t...
09 September 2018 6,985 3 View
i had designed primers for two sequences using blast tools in NCBI but they gave poor amplification lower than the ladder after gradient pcr i want any one to check them and help me in designing...
09 September 2018 7,919 4 View
i do a study for a gene expression in HCC patients compared to healthy person using real time pcr. i have analyzed ct for gene of interest and house keeping gene in each sample either HCC or...
07 July 2018 6,936 13 View
i will do a real time PCR for miRNA after revrse transcription. iam afraid from bad results and wasting mu kits which is expensive. i want to know whether it is possible to do conventional pcr and...
03 March 2018 3,903 12 View
I have used the following procedures but am still having strong primer dimer bands while the desired product is absent. The cycling conditions for PCR program that i used were 5 min at 95C for...
03 March 2018 6,050 6 View
i want to make haplotype and linkage analysis. i found that the most common programme is the haploview. but i don,t know how to make pedigree or linkage or premakeped file to use in the haploview....
03 March 2016 1,013 0 View
i want to choose the green open access because it is free but i want to know every thing about it
02 February 2016 3,163 4 View
I WANT TO DESIGN primers for microRNA
11 November 2015 2,210 1 View
i want to know which method is suitable to extract miRNA from serum or plasma use triazole or total RNA column spin or miRNA column spin isolation kit . many studies used the first first and the...
11 November 2015 7,355 8 View
i want to know what is better for miRNA THE ONE STEP OR TWO STEP PROTOCOL ( ACCURACY AND COST)
11 November 2015 3,301 3 View
i want to know what is better to use real time with sybr green otr taq man for quantification of miRNA
11 November 2015 3,790 8 View
What is better, the use of total RNA extraction kit or mi RNA extraction kit to extract miRNA from serum or plasma . What is better for whole blood?
11 November 2015 5,891 7 View
i want to know the steps for RNA reverse transcription and how i can design its primer
10 October 2015 1,533 8 View
i want to know how to choose the respectable journals without fake impact factor for publication. i depend only on journals under the umbrella of Elsevier-springer- willy network. i want other net...
09 September 2015 3,595 6 View
i want to know about real time pcr primer way of designing. i want the programe used for designing real time pcr primers. i want to know hoe to interpret the results. i want to know how to avoid...
09 September 2015 7,092 14 View
i want a program to convert the Elisa absorbance results to conc
07 July 2015 8,307 10 View
i wnat to understand all the information about this SNP (C667T= c665t=rs1801133). i found several names for this polymorphism. i want to know the cause of choosing the number 667 or 665. i think...
02 February 2015 1,184 2 View
I made a pcr reaction. I want to expect the size of the product recation. I have no reference except the guide paper
12 December 2014 10,051 22 View
i have made a gel electrophoresis for a 7 samples after double arms pcr . the guide paper mentioned that the amplicon is 117 bp but i have seen more than one band in each well
12 December 2014 1,146 16 View
it is my first time to use this technique (tetra-primer arms pcr). i want to know every thing about it. especially the percent of outer primer to inner primer. how can you calculate annealing temp...
11 November 2014 8,202 4 View
Actually, I need this thermal cycler device in my lab. But the faculty budget for the devices this year and the next year is finished. Some colleagues advised me to search for other universities...
11 November 2014 9,392 11 View
Please, I will do a tetra-primer ARMS-PCR experiment. I need any one who has experience in this method to advice me about the work way. I bought primers- master mix- template. I need the...
10 October 2014 1,477 5 View
Read 1 answer by scientists to the question asked by Noha Said on Oct 31, 2014
10 October 2014 2,514 1 View
i need a positive control for my PCR. iam not sure about my samples. is it possible to use the ladder
10 October 2014 5,838 28 View
i want to check the DNA conc. but i don,t know what is the most easy method
10 October 2014 3,739 0 View
I will do a tetra primer ARMS-PCR reaction. I will use four primers for each sample. The four primers are different in melting temperature. I want one annealing temperature suitable for all the...
10 October 2014 748 10 View
I have made a PCR reaction and made a negative control. I found that there is a primer dimerization in most samples. I will decrease the primer concentration but I don't know whether I have to...
10 October 2014 3,608 5 View
I want to know the typical conditions for DNA rehydration. How can I get rid of any remnants of ethanol?
10 October 2014 6,487 8 View
I did a DNA extraction, but I am not sure if the rehydration step was good or not. The kit instructions told me to keep the DNA at 60°C for 90 minutes, but I only kept it there for 30 minutes.
10 October 2014 4,226 24 View
I did a DNA extraction for some blood samples with a ready extraction kit (solution kit, not spin column). Then I ran the DNA on the gel, but no bands appeared. I continued my work and did a PCR...
10 October 2014 10,023 19 View
I have frozen DNA samples and I want to do PCR on them. I want to know what I have to do with the DNA samples after getting them out of the freezer to ensure the DNA is completely soluble in the...
10 October 2014 1,219 14 View
I want to know the difference between PCR-tetra primer ARMS, PCR-AS, and PCR-CTPP. I want to use the most simple one in the genotyping.
08 August 2014 1,390 3 View
I WANT TO LEARN BIOINFORMATICS AND I DON'T KNOW HOW TO BEGIN
08 August 2014 1,594 2 View
I want to know the optimum conditions for having good results for tetra-primer ARMS-PCR
08 August 2014 2,272 2 View
i need to use NCBI for many purposes, but i have a little experience in using it....i need information about some genes, i want to design primer, i want to use the blast tool... i want to do many...
08 August 2014 9,942 4 View
I need the steps for obtaining the sequence for tthe XRCC2 gene in human genome from NCBI.
06 June 2014 8,617 3 View
Seeking to learn the steps for using the BLAST tool in NCBI for designing primers for make a PCR a specific SNP.
06 June 2014 9,398 5 View
