For my cell line study if I use lyophilized form dried powdered plant material instead of normally dried powdered plant materials, what is the benefits I will get from my research investigation? And what type problem I will face if I use normally dried powdered plant materias?? Please share your views.
Lyophilisation, aka freeze-drying, starts with deeply frozen material, which is exposed to a cold trap under high vacuum. Water in the sample sublimates (goes from solid to gas without melting), the heat of evaporation keeps the sample cold. The vapor condenses on the cold trap. This turns the solute material into a very fine, easily soluble powder with little denaturation. Even some proteins survive lyophilisation intact.
If you remove water from the liquid state, say, in a rotational evaporator or spray-dryer, the sample may turn into a shoe-polish like, difficult to dissolve mass, with extensive aggregation of proteins and chemical decomposition of sensitive compounds.
An everyday example of a lyophilised product is instant coffee.
Dear Sir,
Thank you very much for your answer.
But I have question . That is if I keep the extracted material to 37 degree Celsius for few days then is there any possibility to degrade the bioactive compounds?
That depends on the compound and the enzyme set of the plant. If you want to lyophilise, you need to freeze anyway. So why not freeze right away and store at -80 °C?
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