Hi all,
I understand that quantification of total phenolic content (TPC) refers to all the phenolic compounds present in the sample of interest, however, the folin-ciocalteu assay does this by a redox reaction (all reducing substances). On the other hand, antioxidant capacity can be determined by DPPH assay in which the AO reduces the free radicals. What is the purpose of quantifying both TPC and AO capacity?
Are we quantifying both separately because not all phenolic compounds have antioxidant capacity?
*Please correct me if I am wrong.
There are many antioxidants in fruits. Phenolic content does not reflect the total antioxidants in fruits
Kindly check this https://www.researchgate.net/publication/272133306_The_Folin-Ciocalteu_assay_revisited_Improvement_of_its_specificity_for_total_phenolic_content_determination
Also check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5105635/
yes. you can identify separately. you can check antioxidant activity by many models including DPPH, ABTS, DMPD, SUPEROXIDE SCAVENGING, ETC.
Dear Natasha,
The major plant compounds characterized by antioxidant activity are polyphenols. These are present in most plants and are considered to prevent free radicals associated damages in numerous ways including direct scavenging of free radicals and inhibition of enzymes involved in free radical production.
Whereas, TPC activity is the process to figure out the amount of phenolic content present in the samples. Phenolic compounds that contained in the plants have redox properties, and the properties allow them to act as antioxidants.
After checking antioxidant activity in the samples, We perform quantitative assay through total phenolic content, total flavonoid content, and total saponin content, etc. to check the total quantity of secondary metabolites present in the sample.
Regards,
Divya Jain
India
- Badges
- Science topic
- Similar topics
- Agricultural Science
- Environmental Science