Hello
can anyone your knowledge about the difference between the coding potential of human and virus.
Hi, I am currently trying to extract the pesticides fluroxypyr and prothioconazole from the soil by spiking using Dutch Mini Luke extraction method; however, I am failing. The instrument used for...
18 November 2020 4,187 9 View
Hi, I am looking to test the effectiveness of pesticide residues extraction from the soil sample using the Dutch mini luke extraction method. The extraction method would involve the mixing of...
19 October 2020 1,463 4 View
Hello All, I am working on human novel lncRNA expression & quantification in different cell types, I build index files ( hg38) with & without --exon --splice-sites by Hisat2, Is their any...
19 September 2019 7,213 1 View
Hello, I have list of 120 human proteins which is interacted with virus, which is experimentally proved, i want to know about common features, conserved patterns among the proteins Can you...
25 February 2019 3,335 3 View
Hello All I have a time series micro-array data, the format look likes, patient samples collected from Day 1 to Day 40 ( sample size 40)and control sample ( 20). I would like compare all the...
27 December 2017 7,076 1 View
Hello, Presently NGS applied all biological fields, my question is How NGS could use as a diagnostic tool for very earlier and accurate detection of viral diseases. What are all the scope and...
15 June 2017 7,127 2 View
Hello friends I am doing micro array data analysis(HGU1333plus2), i got the expression matrix file by using gcrma , but the some probe is represent multiple gene like this . how can we treat this,...
09 February 2017 3,214 2 View
I would like to develop a hypothesis for my project where i study the difference of soil phenolic content between two different land use types (Primary forest and Oil palm plantation). I want to...
18 May 2016 6,782 2 View
Hello I want to do pathway analysis of list of miRNA which are related to multi drug resistance, can you Share any ideas about the suitable tools?
04 April 2016 340 1 View
Dear All I am concentrating the meta analysis of Microarray data, some of the data set does not have the paper. So give me some suggestions to use those data sets different probe id for the same...
11 March 2016 1,057 3 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...
20 July 2024 1,339 2 View
Hello researchers, I have sequenced the A549 cell line using RNA sequencing approach and then I have it against the human genome reference sequence. Would I expect a 100% mapping percent? I got...
20 July 2024 9,705 0 View
Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...
18 July 2024 4,183 2 View
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
18 July 2024 1,579 2 View
We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...
16 July 2024 118 2 View