Extracting fluroxypyr and prothioconazole from soil?

Hi, I am currently trying to extract the pesticides fluroxypyr and prothioconazole from the soil by spiking using Dutch Mini Luke extraction method; however, I am failing. The instrument used for...

18 November 2020 4,006 9 View

Separation of solvents in Dutch Mini Luke pesticide extraction method?

Hi, I am looking to test the effectiveness of pesticide residues extraction from the soil sample using the Dutch mini luke extraction method. The extraction method would involve the mixing of...

19 October 2020 1,318 4 View

How to compare time series data of gene expression studies?

Hello All I have a time series micro-array data, the format look likes, patient samples collected from Day 1 to Day 40 ( sample size 40)and control sample ( 20). I would like compare all the...

27 December 2017 6,945 1 View

How could NGS act as viral diagnostic tool?

15 June 2017 6,963 2 View

How significant will be difference of soil phenolic content between primary forest and oil palm plantation ?

I would like to develop a hypothesis for my project where i study the difference of soil phenolic content between two different land use types (Primary forest and Oil palm plantation). I want to...

18 May 2016 6,666 2 View

Pathway analysis of miRNA?

04 April 2016 216 1 View

How NGS applied in diagnostic virology field?

Next Generation Sequencing (NGS) is an emerging field applied various biological field. What could be the possibilities of NGS for diagnosing viruses from the clinical samples efficiently and...

01 January 1970 6,775 3 View

How big data methods applied in Bio-Statistics in terms of healthcare and medical data?

Hello everyone, I am very new to this topic, I want to know how big data methods (Hadoop, HIVE, HDFS) applied in Biostatistics. For example to analyze patient data, disease outbreak, trend...

01 January 1970 5,385 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View