Extracting fluroxypyr and prothioconazole from soil?

Hi, I am currently trying to extract the pesticides fluroxypyr and prothioconazole from the soil by spiking using Dutch Mini Luke extraction method; however, I am failing. The instrument used for...

18 November 2020 4,006 9 View

Separation of solvents in Dutch Mini Luke pesticide extraction method?

Hi, I am looking to test the effectiveness of pesticide residues extraction from the soil sample using the Dutch mini luke extraction method. The extraction method would involve the mixing of...

19 October 2020 1,318 4 View

How to compare time series data of gene expression studies?

Hello All I have a time series micro-array data, the format look likes, patient samples collected from Day 1 to Day 40 ( sample size 40)and control sample ( 20). I would like compare all the...

27 December 2017 6,945 1 View

How could NGS act as viral diagnostic tool?

15 June 2017 6,963 2 View

How significant will be difference of soil phenolic content between primary forest and oil palm plantation ?

I would like to develop a hypothesis for my project where i study the difference of soil phenolic content between two different land use types (Primary forest and Oil palm plantation). I want to...

18 May 2016 6,666 2 View

How NGS applied in diagnostic virology field?

Next Generation Sequencing (NGS) is an emerging field applied various biological field. What could be the possibilities of NGS for diagnosing viruses from the clinical samples efficiently and...

01 January 1970 6,775 3 View

How big data methods applied in Bio-Statistics in terms of healthcare and medical data?

Hello everyone, I am very new to this topic, I want to know how big data methods (Hadoop, HIVE, HDFS) applied in Biostatistics. For example to analyze patient data, disease outbreak, trend...

01 January 1970 5,385 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View