I am looking for a protocol which I can get the purified DNA from FTA cards easily and inexpensively.
Then I want to use TE buffer instead of using buffer AT, ATL.
The thing I want to know is that if buffer TE can work instead of buffer AT, ATL or not.
ATL is a lysis buffer which has SDS and EDTA in it and is not recommended for long term storage.
AL has guanidine hydrochloride and malic acid to denature proteins.
TE is Tris/EDTA so uses tris to maintain the pH and EDTA to chelate metal ions which would damage the DNA.
A crude method for getting some DNA for PCR might simply be to boil the cards in some distilled water for 15 mins.
This research gate question covered the functions of the buffers (https://www.researchgate.net/post/What-is-the-exact-composition-of-AL-buffer-of-DNA-isolation-kits#:~:text=For%20buffer%20AL%2C%20it%20says,of%20the%20buffer%20they%20use.)
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