What is the current trending analytes for the micro extraction techniques based on LC-MS/HPLC/GC?

More Muthusankar Eswaran's questions See All

How to coat Graphene on ITO/GC ?

Hello All, I've been trying to coat Graphene on ITO/GC. The problem i'm facing is Graphene is not getting coated on the ITO/GC. What kind of electrolyte? as well applied voltage? i have to use to...

04 May 2018 8,964 4 View

I need to coat graphene on Glassy carbon electrodes for electrochemical studies?

Dear All, I've synthesized Graphene by hummer method and want to use it for electrochemical studies. Since graphene has no functional group in it. How Can I coat it over GC or directly drop cast...

02 March 2018 5,452 3 View

How to stabilize glassy carbon electrode (GCE)? and how to clean it?

Hai All, How to stabilize glassy carbon electrode? During my experiment it is keep on giving noise peak. How can I reduce it. Also how to clean GCE after experiment.

01 February 2018 952 0 View

Solvent to solubilize Diphenylamine?

Actuall I'm doing electrochemical analysis for Diphenylamine. In HCl diphenylamine's solubility is very less. What are all the other solvent I can use for this purpose?

01 February 2018 9,383 3 View

Hai all, Can anyone tell how to make a gas sensor in laboratory (simple setup)?

09 October 2016 1,042 4 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

Isam Eldin Hussein Elgailani

Please look at the following below links which may help you in your analysis:

https://www.tandfonline.com/doi/pdf/10.1080/10408340590947952?needAccess=true

https://pages.pomona.edu/~wsteinmetz/chem160/spme.pdf

http://www.chromatographyonline.com/microextraction-and-its-application-forensic-toxicology-analysis-0

Thanks

Rafea Naffa

Use SPME and use the right coating for your application

Grzegorz Boczkaj

Deep Eutectic Solvents.

Chaeck my papers.

Regards