Current knowledge of PK/PD of polymyxin B shows cellular apoptosis of renal epithelium leading acute tubular necrosis. Should we load dose in treatments with this antimicrobial agent?
When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...
27 February 2021 6,326 2 View
I need a free of charge and short questionnaire that measures mood states. The questionnaire will be used as in a neuropsychological evaluation study. It is well known that mood states can...
16 January 2021 8,284 4 View
I need to compare the behavior of evolutionary optimization algorithms on well-established benchmarks such as CEC-2013 or psplib, based on the existing literature. One difficulty is that there is...
18 September 2020 6,812 3 View
I have a small dataset and need to learn a mixed probabilistic model (discrete + continuous) and simulate new values taking into account the learned structure.
18 September 2020 8,709 4 View
I am writing an article on #thedress. I have read that this illusion is partially explained by daylight locus, and I do not fully understand this idea. I know that it represents the distribution...
11 September 2020 8,645 1 View
The sensitivity of the technique is important to know how to identify the appearance of cracks
17 August 2020 4,156 9 View
I'm measuring the phosphorylation status of different proteins (e.g. NFKB, ERK, AKT1, p38 etc) using phosphoflow cytometry. The protocol for surface and intracellular staining works well, all the...
22 July 2020 3,233 8 View
How to calculate R_0, R_t, beta (the average number of contacts per person per time) and gamma? I try to defined SIR model, my data have infected, death and recovered. My country show the next...
09 April 2020 8,237 4 View
Dear all We have uploaded a preprint of our paper about COVID-19 modeling. Understanding COVID-19 spreading through simulation modeling... Any researcher interested in giving us feedback?...
30 March 2020 2,232 5 View
I'm having trouble finding information on this because I am not familiar with the nomenclature for graph theory. If you don't know of a specific algorithm, or if you know no such algorithm exists,...
29 February 2020 7,289 3 View
Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...
22 February 2021 4,032 4 View
I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...
16 February 2021 169 4 View
I am looking for PCR primers for bacillus Sp.
15 February 2021 2,552 5 View
I know that efficiency of transfection for primary B cells is very low with Lipofectamine. But I did not try that for EBV immortalized B cells. Do you have any experience with transfection of EBV...
05 February 2021 4,159 1 View
The bacterial NRPs (nonribosomal polypeptides) are famous as clinical drugs such as antibiotics, but is there other native function of these NRPs of bacteria except antimicrobial, because their...
01 February 2021 4,280 1 View
I was wondering if euthanizing mice by carbon dioxide inhalation would cause activation of NF-kB inflammatory response?
26 January 2021 4,096 2 View
Hi everybody, I am setting up a method for analysis of colistin sulphate by HPLC-UV. I tried different previously-published methods but the results were not acceptable (long run time, bad...
16 January 2021 5,911 3 View
I have designed a primer for Bacillus subtilis sfp gene. As I have mentioned the size is 191 bp. however I need some suggestions regarding PCR temperatures and cycle length. As the it is smaller...
09 January 2021 8,309 4 View
Even if I get them cultured colonies are very less and almost not transferable and grows very slowly in the media. I checked cells by transforming with control vector. I changed antibiotic also...
06 January 2021 1,013 3 View
It is known that scientific knowledge is replicated each time at more unusual speeds, however, humanity expects more from our scientific work, that is, solutions to problems that have accumulated...
02 January 2021 8,300 16 View