Current knowledge of PK/PD of polymyxin B shows cellular apoptosis of renal epithelium leading acute tubular necrosis. Should we load dose in treatments with this antimicrobial agent?
Census data seems only to reach the year 2011, but there might be estimates somewhere that I cannot find? I am looking not only for the largest cities but also towns etc. Thanks!
10 August 2024 857 4 View
Cuáles fueron las tendencias en investigaciones en arquitectura, urbanismo y patrimonio edificado en decadas del 2000 al 2020? Porque requiero conocer tesis de posgrado nivel maestría...
24 July 2024 5,494 1 View
I am trying to do Grounded Theory in documentary research. I have obtained the most frequent terms (induction) to perform deductive coding and so, articulate a hypothesis about the hidden...
05 June 2024 2,599 2 View
I am using SU-8 2025 to generate a high aspect ratio microchannel. I made two microchannels with the properties of: 1. width = 10 um. height = 25 um. SU - 8 2025. 2. width = 15 um. height = 37.5...
27 May 2024 6,862 2 View
The yeast K. marxianus is well known for its ability to ferment de disacharide lactose. It is also able to ferment the disaccharide maltose or not? If you can share citations with respect to this...
19 May 2024 7,309 3 View
Greetings community. Are there any experts in this topic you can recommend to build a solid theoretical revision for a reflection around the topic? Thanks for your help.
10 April 2024 1,592 2 View
I have screened a series of synthetic compounds on cancer cells, and some of them were contaminated. These compounds were diluted in 100% DMSO. With a low volume of the compounds (800-1000 µl), I...
25 March 2024 4,740 0 View
Teoric project for IB Biology Internal Assesment
06 March 2024 5,061 1 View
Dear colleagues, I hope this message finds you well. In our laboratory, we routinely conduct primary neuronal cultures derived from embryonic cortex, hippocampus, and spinal cord. Generally, our...
10 December 2023 1,883 1 View
The unequal distribution of water resources in the world and the impact of climatic changes.
22 November 2023 9,926 3 View
Can someone explain to me how was the MIC of MO in combination and MIC of antibiotic in combination was calculated? as shown in the attached file below.
26 July 2024 2,702 1 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
In reference to antibiotic as a commodity, what is your take and way forward on common molecules in both Veterinary and Human antibiotics in relation to currently experienced emergency of AMR?
20 July 2024 4,020 0 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...
11 July 2024 7,847 1 View
During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...
08 July 2024 1,330 0 View
I'm going to transfect cells with expression plasmids and later siRNAs against the same gene. Is it true that penicillin/streptomycin in the media negatively affects these processes? Shout I use...
07 July 2024 8,671 6 View
I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...
27 June 2024 2,951 5 View
Looking for a protocol for the stimulation of B cells with pokeween mitogen
22 June 2024 4,882 4 View
In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...
19 June 2024 7,822 3 View