Dear colleagues,

I hope this message finds you well. In our laboratory, we routinely conduct primary neuronal cultures derived from embryonic cortex, hippocampus, and spinal cord. Generally, our cultures thrive, displaying robust growth, well-defined projections, and established connectivity. However, we have encountered a peculiar issue, which is emerging approximately 7-10 days post-plating.

The specific structures we observe during this time frame raise concerns, and we are reaching out to you all to inquire if others have experienced similar challenges and, if so, how they have successfully addressed them. Any advice or insights you could provide would be greatly appreciated.

For context, our culture protocol is outlined as follows:

If you have encountered and successfully resolved similar issues in your primary neuronal cultures or if you have any suggestions on troubleshooting steps, we would be extremely grateful for your guidance.

Thank you in advance for your time and consideration.

Best regards,

César O. Lara