What is the culture media for LX-2 cell line? I am using DMEM supplemented with 10%FBS and 1%P/S. It has been more than a week but cells confluency in 100 mm dish is only around 20-25%?
You can use RPMI1640 supplemented with L-glutamine. Use this medium to prepare a 5% NBCS, 2% antibiotics (penicillin, streptomycin and amphotericin B).