i prepared by mixing of drug solution into antisolvent containing polymers, the zeta potential slightly changes, can i say the drug nanoparticles encapsulated into polymer ? if not why and then what will be the reliable explanation for it.
There are many effects that influence zeta-potential, that's why its slight changes (in my opinion) have no meaning. It happens all the time. Slight changes of zeta-potential can easily happen because of the change of solute concentrations. So when you add something to your suspension (e.g. you added polymers to it) it has to change anyway. The question is how much it changed and how can you rule out effects that may have caused this change.
Vamshikrishna Karanam , if he do what u say he will assay only the amount of the drug that will be separated and leak to the supernatant but are u sure that there will no drug inside the nanoparticles after the centrifugation remain in the inside it ?
Hai vamshikrishna karanam, thank you for describing the procedure for EE % calculation, could i go for calculation of Drug Loading % also, by following formula
DL%= [ total amount of drug - amount of drug in supernatant] / [ total amount of drug+ total amount of polymer ]
but the Q is some amount of polymer was dissolved in solvent ( Bcz the solvent is solution of polymer).
Dear All
I want to know that the precipitated material may be the entrapped drug but I think we cant say it encapsulation, as the particles suspended may be in agglomerated form. You must go for the SEM and TEM both for the final decision.
Dear Raj
if you go for TEM then you will get the image as attached the coated polymeric layer defines that is is an encapsulated product.
Thank you Anuj Mittal,
very Very much Thanx, i never try to this during TEM, FESEM. i will try, at which KV u r done TEM imaging and with stainied or without staining,? bcz organic compounds (drugs) may get decompose in TEM due to high voltage.
i done with staining of PTA 2(w/v)% aqueous solution and at 50 KV,
if there is a good procedure for sample preparation and instrumental condition suggest me.
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