Which book is the best to follow for doing qualitative research ?

I am doing social constructionist research on everyday life. There are so many books on qualitative research that it is hard to decide which one is among the top best ones. I am searching for a...

12 May 2020 328 3 View

Statistics of histology scores?

Hi, (a) I am trying to analyze histological scores of more than 3 groups, each having 6 animals. Which statistical tool is the appropriate one? I checked normality which appears to be of mixed...

16 January 2020 4,661 3 View

Cytotoxicity assay from culture supernatant?

Hi, I was planning to perform a cytotoxcity assay wherein I am planning to use the culture supernatant stored frozen at -80 deg C at different time points. Is there any suggestion with regards to...

18 November 2019 3,724 1 View

Detection of Rat X and Y chromosomes by PCR?

Hi, I was wondering if anyone has the protocol for rat X and Y chromosome detection by PCR particularly Digital PCR. I want to quantify the actual amount of X and Y chromosome from the same...

14 October 2019 9,257 3 View

RNA stability at different temperature?

Hi I was wondering if any one have checked RNA stailbity at different temperature for subsequent PCR amplification. I want to store my RNA isolated from blood vessels before preparing the...

17 July 2019 3,541 12 View

Macrophage culture from rat?

Hi, I want to isolate and culture macrophages from rats (different sites like bone marrow, peritoneal fluid etc.) Most of the reported literature says to use the supernatant from mouse fibroblast...

11 July 2019 7,314 3 View

Can any one please provide me a protocol for how to extract and purify alpha amylase from animal pancreas?

I want to extract alpha amylase from goat pancreas. And alpha amylase and alpha glucosidase are same enzymes?

01 May 2019 6,151 3 View

Why male Wistar rats are preferred over females in diabetes research?

I am a research scholar , conducting research on antidiabetic potential of herbal compounds. I have found that in vivo testing of anti diabetic drugs are mostly done on male wistar rats. So,...

01 November 2018 2,199 6 View

I am looking for a simultaneous heat and mass transfer model with finite differences?

I am trying to come up with a code for simultaneous heat and mass transfer for water evaporation owing to solar flux. The incoming heat flux contributes to volumetric heat generation within a...

24 October 2018 8,964 1 View

How does infrared absorption peak of carboxylate (COO-) change with metal attachment?

My polymer shows symmetric and asymmetric peaks at 1421 and 1605 cm-1. The polymer was able to reduce palladium nanoparticle. If carboxylate is responsible for reduction then Which direction...

14 May 2018 6,138 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How UAV or drone can be beneficial to better estimate crop yield?

As we know, crop yield estimation has been done traditionally with farmer's expert knowledge, deductive sampling in representative areas, and remote sensing with satellite imagery. Farmer's Expert...

01 March 2021 3,468 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View