Hi,
I am wondering about the following things while desgining a digital PCR expt-
(1) Do we need to run the test and reference gene in the same well?
I found an interesting aricle where this has been tested-
Mitigation of the effect of variability in digital PCR assays through use of duplexed reference assays for normalization
(2) If we have low RIN value for any sample can we trust the data obtained from digital PCR assays?
Thanks.