Can anyone tell me the relationship of gene expression in base pairs and amplicon size?

I wish to know is it possible to correlate the expression of a gene in base pairs with its amplicon size.

09 October 2017 6,242 0 View

Can anyone suggest me a program which can be used to check whether a particular gene sequence may be working or not?

10 November 2016 5,294 7 View

Did anyone know whether Heart type fatty acid binding proteins and Liver fatty acid binding proteins are present in HL-60 cells?

I am working on some fatty acids. I wish to know whether FABPs of Heart and Liver types are present in HL-60 cells.

08 September 2016 9,216 1 View

Can anyone suggest me an useful software that can be used to check whether a given DNA sequence is working or not?

As part of my research work, I have to check the expression of some genes. However when I go through references, I got different sequences for the same gene. I wish to know which of these...

08 September 2016 5,137 4 View

Can Propidium Iodide uptake assay be done in rat heart/ liver tissues?

As part of my research work, I have to perform apoptosis assay with animal tissues. I wish to know, whether Propidium Iodide uptake assay be done in rat heart/ liver tissues.

07 August 2016 6,155 3 View

Can anyone suggest me some good kits for performing TUNEL assay in vivo?

As part of my research work, I have to perform TUNEL assay in in vivo. Can anyone suggest me some good kits which are economical, based on their experience?

07 August 2016 2,971 2 View

Can anyone provide me the calculation for SOD using the Method of Kakkar et al., (1984)?

I am working with the effect of certain chemicals in antioxidant system using Cell line models. As part of my work, I have to perform SOD assay. I choose the method of Kakkar et al., (1984), since...

06 July 2016 3,290 1 View

Can anyone please suggest to me a method for isolating cell membranes?

I am working with cell lines. As part of my work, I wish to isolate the membranes of cells. I wish to know if there is any simple method for cell membrane isolation which can be done without using...

02 March 2016 6,495 1 View

Is there any simple method which serves as a marker assay for inflammation instead of COX-2 and LOX-5?

Hello, I am working on cell culture. I wish to know if there is any simple method which serve as marker of inflammation instead of Cyclooxygenases and lipooxygenases.

11 December 2015 6,901 4 View

Is it suitable to carry out centrifugation in normal centrifuges (instead of cooling centrifuge) while doing enzymatic assays?

Hello, I wish to know Whether it is good to carry out centrifugation in normal centrifuges instead of cooling centrifuges while doing enzymatic assays especially in cell culture.

10 November 2015 8,195 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View