What is the concentration of PBS solution used in MTT assay?

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Saba Tabasum

its 1x PBS

Santosh Karnewar

1x dpbs also we can use

Dmitry B. Kazansky

If you see precipitation after dissolving MTT in PBS, try to use 0.9% NaCl.

Uzair Hashmi

@amit ,

we have to keep equal volume during our experiments, so in the negative control or the untreated cells, we have to equate the volume with 1X PBS. Let me know if my answer needs further elaboration.