How can we measure the wear resistance and toughness for steel wire?

I want to know different testing methods to investigate the mechanical behavior of steel wire

29 December 2020 610 1 View

Or can the Kalman filter be used for medical image processing ?

Or can the Kalman filter be used for medical image processing ?

30 November 2020 2,096 3 View

Sterilization of animal serum to be used for blocking in immunohistochemistry ?

Hi everyone, In case that, it is difficult to get animal serum from commercial vendors. Have you ever tried to collect animal serum to be used for blocking in immunohistochemistry ? If so do we...

04 January 2020 3,637 5 View

Can anybody share a solidification model file for any alloys (ANSYS / COMSOL Multiphysics) for a simple configuration (rectangular)?

in solidification of an alloys in comsol there is problem to control concentration of an alloys in Mushy zone And also how do I associate between heat conversion and Transport (ht) of...

18 October 2019 4,023 3 View

Co-simulation PSIM_Matlab is there any chance to use new version of matlab?

Generally, co-simulation PSIM MATLAB 2010 is already works. But with new version of matlab from 2013, ... To 2019 here fail to work. Any contributor in this problem?

29 July 2019 3,958 3 View

What is the role of clusters in sustainable energy development?

Currently, the global priority is the implementation of sustainable energy development. What is the role of clusters in this regard?

15 April 2019 5,668 4 View

How to be defined the thermodynamic magnetic field Hc from curve M(H).

This figure shows the M as a function of H at T fixed.How to be defined the thermodynamic magnetic field Hc from curve M(H).

21 January 2017 8,805 4 View

How can i repare an Ag/AgCl reference electrode ?

15 October 2016 8,015 11 View

What are the best way to remove the polystyrene (PS) beads which coated with a metal (Ag, Au or Pt).

30 August 2016 8,105 4 View

Is there any way to prepare my own AIN-93M diet for mice?

I need a AIN-93M diet for rodents, how can i prepare it ? especially the vitamin mix and mineral mix. regards

29 March 2016 1,742 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Should GFP sequence be cut together with the IRES sequence?

Hello. I have a viral plasmid which contains a bicistronic region: CMVprmtr-GFP-IRES-PuroR. I would like to cut out the GFP sequence to get rid of the green fluorescence. In this case, would it be...

02 February 2021 1,644 7 View

How to do DNA normalization based on qPCR values?

Hi Everyone: I have a question regarding to qPCR. I am trying to do RT-PCR, and I want to have equal amount of input DNA for my RT-PCR, so I normalize my DNA input based on qPCR values of a...

31 January 2021 6,977 4 View

What is the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow endothelial cells?

Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...

28 January 2021 8,765 4 View