What is the composition of Vector M.O.M. Blocking Reagent?

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ANSYS FLUENT is only launching in parallel. Can somebody please help?

My fluent is not launching in series at all, even when I uncheck the parallel option and i also have no option for using series. Please help!!! Many thanks!

04 September 2020 4,199 4 View

I am trying to simulate an electric arc formed because of current flowing through air between 2 conductors in ANSYS Fluent. Can i use MHD module?

If i use the MHD module, i will have to provide the values of external magnetic field, but isn't the magnetic field established as a consequence of the current flowing through the air? How am i...

15 August 2020 200 1 View

Removal of dead cells before harvesting for Proteomics?

Dear all, I am culturing some suspension cell lines. I am planning to perform proteomics (LC-MS/MS) experiments on them. Unlike adherent cells (viable cells are adhering to the substrate and...

03 March 2020 2,329 3 View

Enrichment of AHA-tagged proteins: Should I use DBCO- or Alkyne-based methods?

Dear colleagues, I would like to seek your kind advice on click chemistry enrichment of azidohomoalanine (AGA)-tagged proteins. From literature, I learned that there are 2 main avenues, either...

18 February 2020 8,725 2 View

Why do you need to preheat antigen retrieval buffer?

I'm troubleshooting immunofluorescence staining for some antibodies. Some work fine with my protocol [H2AX, Ki67, 3-nitrotyrosine], some stained once and never again [8oxoG], and some won't stain...

21 April 2019 5,510 3 View

What is the ideal activation time for stain-free gels?

From Bio-Rad's user guide (see below) it is not clear to me what is the best activation time to use. It states that 1 min gives optimal results for WB followed by immunodetection, but that 5 mins...

25 March 2019 1,508 4 View

Who knows a good surgical monitoring system for mice?

I am looking for a good surgical monitoring system for mice, mainly ECG recording. For different suppliers it is mostly the same Scintica Rodent Surgical Monitor+ system. Does anyone know a...

02 March 2019 5,069 1 View

Who knows a good surgical monitoring system for mice?

I am looking for a good surgical monitoring system for mice, mainly ECG recording. For different suppliers it is mostly the same Scintica Rodent Surgical Monitor+ system. Does anyone know a...

28 February 2019 2,000 1 View

What is the composition of Vector M.O.M. Blocking Reagent?

Has anybody an idea what might be in the Vector M.O.M. blocking reagent? The kit works very well in background reduction and one could use rabbit antibodies on rabbit etc. if we would know the...

02 December 2018 7,486 3 View

What statistical analysis is recommended to compare profiles?

I am looking to compare two cultural groups (independent variable) and their resilience profile (dependent variable). There are six factors within the profile, so I feel that a MANOVA would be...

14 November 2018 8,566 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Don Kaiser

Great question! It's a shame scientists don't even know what they're doing any more with all of these proprietary kits.

Byron Hsu

The M.O.M. Blocking Reagent blocks endogenous mouse Ig using a different method from monovalent Fab fragments. It should be used for mouse antibodies only on mouse tissue.