16 November 2019 4 1K Report

This is the upper part of a western blot membrane, and as you can see there are some proteins that did not enter the separating gel.

Does this affect the level of protein expression? I mean is it wrong to take the lower band as a final result ?!

What could be the problem, and how to fix it?

Is it because I've loaded a hight amount of protein (60ug)?

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