How to interpret different leached phases of metapelites?

Extraction of three different phases of metapelites, mostly phyllites as the Carbonate phase, Fe-Mn Phase, and Silicate phase has been obtained. After doing ICPMS, different results have evolved....

11 February 2021 5,198 10 View

Is anyone working on measurement of globalization?

I am in need of the help of anyone who is working on measurement of globalization? Please inform me at the earliest.

23 January 2021 1,259 1 View

Whether new variant will affect the diagnostic method of detection or not?

can it be diagnosied by the same tool or different tool

04 January 2021 2,964 2 View

Python modules for old Spectrum-Analyzers?

Hi All, Are there any python modules available for old HP-Agilent spectrum analyzers around? I know PyMeasure has a library of some commonly used and new electronics but I am looking for old ones...

21 December 2020 3,460 3 View

What should be the ideal voltage and current to scan mice femur and tibia using micro CT instrument ? How these parameters affect the image quality ?

I want to scan my experimental mice bone samples using micro CT instrument. I have gone through several research articles for ideal scanning parameters like voltage, current, filter etc. but these...

11 October 2020 8,069 2 View

How to calculate the defect charge formation energy for a metallic system (bulk/surface)?

How to calculate the defect charge formation energy for a metallic system (bulk/surface) using DFT? The defect formation energy is calculated by the following formula E_formation (D,q) = E (D,q)...

23 August 2020 6,047 4 View

What is form factor of a compact antenna?

How to determine the form factor of a miniaturized antenna?

18 February 2020 882 3 View

What does it mean by energy band convergence in semiconductor by doping?

If band structure is anisotropic in k-space, then how will the convergence of two bands normally appearing at two different symmetric point in the Brillouin zone will enhance See-back Coefficient?

04 February 2020 1,916 3 View

What is the standard procedure of seaweed sample preparation for SDS-PAGE?

Hi everyone... For SDS-PAGE sample preparation,I used 250 microlitres of a seaweed sample with 250 microlitres of treatment buffer. But I didn't get any band/protein fraction in it. So can anybody...

29 January 2020 633 2 View

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06 September 2019 8,466 10 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View