I want to design experiments to study protein-protein interaction in mice.
I am getting a significant marker trait association for traits. But understanding the allelic association is difficult; NN is the missing allele. Can anyone help me?
13 May 2024 8,860 2 View
I have performed the temperature dependent dielectric measurements;with proper checking of all electrical connections of electrodes, sensors,heater etc.as well as their proper resistance of...
07 April 2024 7,625 1 View
I am looking forward to know the concept of Minkowski functional and its geometry to construct a semi-norm? Can anybody suggest me with some good soft copies?
05 March 2024 5,234 2 View
Please send me some standardized manual protocols for the extraction of RNA from Streptomyces sp. Thank you in advance.
18 February 2024 7,298 2 View
A drug showing efficacy in humans when administered through oral route, but the same drug is lacking efficacy in mice admistered through same route. What can be the possible reason?
08 January 2024 4,189 3 View
Trying to find the relationship between epigenetic and cancer more deeply.
30 December 2023 8,363 2 View
The background of my research topic is : I am doing research on a "community tourism" project which started in the year 2016 by the state government of Odisha. Odisha is one of the states in...
18 November 2023 1,292 2 View
How are the shells useful in core@shell nanostructures for energy storage purposes? Can thickness of shell play a vital role for storing energy? Is there any relations between thickness of outer...
16 October 2023 9,275 1 View
Some polymers having low dielectric constant used with high dielectric ceramics. But why we are merging these two high and low dielectric materials?
16 October 2023 2,170 3 View
I have collected data through survey from in 5 point likert scale where 1 as Strongly Disagree to 5 as Strongly Agree. I have total 20 questions in 4 constructs: economy, psychology, political...
08 October 2023 8,723 8 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View