How to interpret different leached phases of metapelites?

Extraction of three different phases of metapelites, mostly phyllites as the Carbonate phase, Fe-Mn Phase, and Silicate phase has been obtained. After doing ICPMS, different results have evolved....

11 February 2021 5,198 10 View

Is anyone working on measurement of globalization?

I am in need of the help of anyone who is working on measurement of globalization? Please inform me at the earliest.

23 January 2021 1,259 1 View

Whether new variant will affect the diagnostic method of detection or not?

can it be diagnosied by the same tool or different tool

04 January 2021 2,964 2 View

Python modules for old Spectrum-Analyzers?

Hi All, Are there any python modules available for old HP-Agilent spectrum analyzers around? I know PyMeasure has a library of some commonly used and new electronics but I am looking for old ones...

21 December 2020 3,460 3 View

What should be the ideal voltage and current to scan mice femur and tibia using micro CT instrument ? How these parameters affect the image quality ?

I want to scan my experimental mice bone samples using micro CT instrument. I have gone through several research articles for ideal scanning parameters like voltage, current, filter etc. but these...

11 October 2020 8,069 2 View

How to calculate the defect charge formation energy for a metallic system (bulk/surface)?

How to calculate the defect charge formation energy for a metallic system (bulk/surface) using DFT? The defect formation energy is calculated by the following formula E_formation (D,q) = E (D,q)...

23 August 2020 6,047 4 View

What is form factor of a compact antenna?

How to determine the form factor of a miniaturized antenna?

18 February 2020 882 3 View

What does it mean by energy band convergence in semiconductor by doping?

If band structure is anisotropic in k-space, then how will the convergence of two bands normally appearing at two different symmetric point in the Brillouin zone will enhance See-back Coefficient?

04 February 2020 1,916 3 View

What is the standard procedure of seaweed sample preparation for SDS-PAGE?

Hi everyone... For SDS-PAGE sample preparation,I used 250 microlitres of a seaweed sample with 250 microlitres of treatment buffer. But I didn't get any band/protein fraction in it. So can anybody...

29 January 2020 633 2 View

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06 September 2019 8,466 10 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View