Will fixing and staining of the cells before diffusion assay affect the results so much?

Hi all, I am going to do a diffusion assay using FITC. My previous results were not illustrative. So, I think if I could stain it with DAPI first it would be more illustrative but I was wondering...

07 September 2020 3,063 3 View

What techniques can we use to reduce the number of items?

My questionnaire is about tracking survey of the higher graduated and it includes 4 sections and 163 items. In each section the nominal, dichotomous, interval and Likert scale is specified. We...

07 November 2018 4,179 3 View

Has anybody transfected an integrative plasmid DNA into rat bone marrow-derived mesenchymal stem cells by lipofectamine 2000 with a high efficiency?

I am working with rat BM-MSCs, and i am trying to transfect these cell with integrative pIRES-EGFP plasmid by applying lipofectamine 2000 (invitrogen). however I found these cells very hard to...

31 August 2018 9,851 3 View

Is the total strain energy vs. tensile and compressive strain symmetric?

Is the total strain energy vs. tensile and compressive strain symmetric?

11 August 2018 9,766 5 View

Is it necessary to determine goodness of fit for Structural model?

Dear Researchers and Experts, I would like to know while, we carried out the the assessment of model fit on the Measurement model, why it is again necessary to determine goodness of fit for...

01 January 1970 8,307 5 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

What is suitable temperature at reaction mixture of lipase activity assay?

I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...

04 February 2021 9,100 5 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

Can you use MTS assay with cells cultured in media containing phenol red?

I am trying to run a cell viability assay with LN229, but these cells are cultured in DMEM which contain phenol red. Is the MTS assay sensitive to the additional of phenol red in the media? If so,...

03 February 2021 9,478 2 View

How to Extract DNA from dried rhizomes, wood, galls etc. stored for a long time?

Dear Respectable Scientists, I have been trying to extract DNA from above mentioned sources using different CTAB/SDS Buffer Compositions and purifying agents but I am not able to extract DNA from...

12 January 2021 5,899 8 View

How to get phenol(BHT) back to organic phase from acidic aqueous layer?

07 January 2021 6,527 3 View

How to desinfect a liquid nitrogen tank?

Hey everyone, we'd like to clean and decontaminate our liquid nitrogen tank before stroreing new cells inside. The tank seems to be made of aluminum. Any recommendations on what disinfectant to...

07 January 2021 1,599 3 View

How can i detect the phenol red in mixture solution?

Hi. I'm trying to detect the phenol red in the mixture solution by HPLC. As you know, It is using for ph indicator. The problem is.. After making phenol red solution in 50 ml conical tube, I...

13 December 2020 5,511 4 View

Does phenol red quench the light emitted by the scintillator in radio active assays?

I would really appreciate it if someone has experience with radioactive assays and could help me troubleshoot my experiment a bit. I could not find any literature evidence, but I am suspecting...

10 December 2020 4,810 3 View

Hi, I would like to test different disinfectants and its effects on biofilms in microplates, which paper has the best protocols!?

I need to do it only in microplates and read the OD value for the results. I cannot use any other assays except microplate. Please help me with this

07 December 2020 5,698 3 View