Hey everyone. As you know, there is a back-order issue with Matrigel, and I was considering substituting Matrigel with Geltrex. Based on what I've found online, the essential factor is adjusting the concentration of Geltrex to match the proper Matrigel concentration. Matrigel COA just mentions the dilution factor, not the protein concentration. Is it possible to reverse-calculate the protein concentration based on the dilution factor? I want to use Matrigel protein concentration to adjust Geltrex dilution for cardiomyocyte differentiation.
Hey Reza,
Is this for coating plates for your cultures?
I have used the recommended concentrations of Matrigel and Geltrex interchangeably for both iPSCs and neurons (Cortical and Motor), and they have been fine.
Their composition is so similar i think the dilution probably will not matter. But you could do this if you wanted to with media.
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