What is the BrafV600E mutation rate of the HT-29 cell line?

13 September 2017 1 945 Report

Hi, every body.

I've got a HT-29 cell line here. I do pyrosequencing to detect the Braf V600E1 mutation rate and get about 30%. Is this possible?

Thanks.

Qi Niu

Thank you, Ajit.

Badges
Science topic

Similar topics
Comment

More Qi Niu's questions See All

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Why PLLA deposited by gap electrospinning lose piezoelectricity?

I tried to prepare aligned PLLA nanofibers by gap electrospinning method. But after the deposition, when I tried to measure its piezoelectric sensitivity, it actually did not exhibit any...

17 June 2024 9,763 0 View

Is there any protocos to produce the medium for single hybridoma cell culture without the feeder cells layer in the plate(Co-culture)?

20 May 2024 8,214 2 View

How about the efficiency of immunization with mRNA on mice or rabbits?

The development of mRNA vaccines on mice and rabbits?

18 May 2024 7,475 2 View

The position of the spots change during movie recording using prism-type TIRFM?

I tried to image Cy5/Cy3 labeled DNA using prism-type TIRFM. But the position of the spots seem to change during movie recording. I think I didn't touch the sample stage of the microscope. I have...

21 April 2024 3,675 0 View

Is this behavior encouraged in your country or culture when someone interrupts you while speaking?

Research indicates that in English-speaking cultures, people welcome interruptions during informal conversations, seeing them as a form of active participation. However, in Japanese culture,...

14 April 2024 183 2 View

I added 3mass% ethanol to PET/HFIP solution and carried out electrospinning, but the nanofiber I got have beads clumped together. Is this normal? Why?

I added 4mass% also get the results of beads clumped together. Is it because the ethanol does not mix well with PET/HFIP?

29 March 2024 2,164 0 View

When is the corrosion tendency greater, at a positive or negative corrosion potential?

In some literature, it is mentioned that the closer the corrosion potential is to the negative direction, the lower the tendency for corrosion. However, in other literature, it is stated that the...

03 March 2024 860 2 View

When an electric current passes through a ferromagnetic material, what is the generating mechanism of the magnetic field in the material?

Does this change the spintronic state of the Fe atom? Or a change in the magnetic domain?

24 February 2024 6,631 1 View

No ion spray current on TSQ Quantis Triple Quadrupole Mass Spectrometer?

I changed the H-ESI needle on TSQ Quantis Mass Spectrometer and found the ion spray current turned down to zero. It was around 31 uA before the change. Does anyone know what caused this issue and...

18 January 2024 5,242 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

Creating an Automaton/Using Language as the Model?

As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...

31 July 2024 9,859 0 View