Any useful source to be prepared for a system engineer/instrumentation engineer role in a X-ray vendor company like Rigaku?

Hi, I'm a graduated Ph.D. trying to land a system engineer/instrumentation engineer position at an X-ray vendor company such as Rigaku and I have some questions about this: 1. How to standardize...

25 October 2020 7,673 4 View

Can one find direct and elementary proofs for two integral formulas?

In the preprint W.-H. Li and F. Qi, A further generalization of the Catalan numbers and its explicit formula and integral representation, Authorea Preprints (2020), available online at...

24 August 2020 4,391 21 View

How to read .msp spectral library in R?

Hello everyone, Does anyone know how to read .msp files in R? I recently obtained a NIST library in MSP form by lib2nist. However, there is no retention indexes information in the MSP library. I...

25 February 2020 7,630 2 View

Surface-based cohesive behaviour: ABAQUS properties?

URGENT! Dear all,I'm currently modelling the masonry wall in ABAQUS using simplified micro-modelling approach. My model didn't go really well, I believe could be something to do with the...

27 July 2019 5,425 5 View

Why my primer efficiency was very very very high?

i am doing primer efficiency test, and i do 1:10 dilution for cdna some efficiency was more than 100, like 178%, however, some efficiency was like 50000000, i never see the efficiency shows 100%,...

06 April 2019 1,354 3 View

What is the optiminization strategy for cardiomyocytes cell culture?

Dear friends, We are trying to optimize the culture condition for human cardiomyocytes. We just want to know if there is an established optiminization strategy for tuning the parameters, such as...

18 September 2018 6,057 1 View

Are there some test electric power systems with geographic location information of the buses?

The IEEE test systems usually do not have geographic information of the system. Does anyone know some test power systems that do have he geographic information of the buses?

03 June 2018 8,908 3 View

Can the methylation level of cultured cells/extracted DNA change?

15 November 2017 1,197 4 View

I want to repeat the COMSOL simulation in water imbibition in a paper matrix using the Richard's equation. How can i simulate the water front?

I want to repeat the COMSOL simulation in water imbibition in a paper matrix using the Richard's equation as reported in Two-dimensional model of imbibition into paper-based network.... When I...

15 November 2017 6,347 2 View

How to prevention and cure the damage by aphid in plant growth room?

29 June 2017 742 31 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View