5 Questions 9 Answers 0 Followers
Questions related from Qi Niu
Hello, does anyone know that if Tween-20 can be used in antigen retrieval buffer in IHC experiment? And what concentration is best? The antigen retrieval buffer I’m using now...
25 February 2019 3,032 2 View
Hello everyone, Recently I tested two different primary antibodies for PDGFRA in carcinoma tissues (breast, colon, ovary) and both showed obvious staining in cell nucleus. However I...
28 December 2017 6,046 0 View
Dear all, can anyone tell me that 1. if the methylation level of some certain genes can change in cultured cells（without any special treatment ) or in stored DNA sample? 2. If the methylation...
16 November 2017 1,341 4 View
Hi, every body. I've got a HT-29 cell line here. I do pyrosequencing to detect the Braf V600E1 mutation rate and get about 30%. Is this possible? Thanks.
13 September 2017 963 1 View
Hi, everyone. I am performing an ARMS Q-PCR for KRAS mutation detection and using the taqman probe. I wonder if the probe need to follow the ARMS primer or not? The probe I use now is following...
24 May 2017 8,598 0 View
