Hi -
We have single stranded genomic DNA from a Chelex-based extraction of dried blood spots. They are extracted into Tris (pH 10).
We would will be utilizing these samples for down stream qPCR within a few months time.
Is it better to leave them at 4 degrees or put them them at -20 but have to take them through a freeze thaw cycle?
I know dsDNA can be stored for long times at 4 degrees, but not sure about ssDNA. Should be long pieces, as genomic, not a cDNA library.
Thanks,
Whitney
Katie A S Burnette
I always store my DNA for qPCR in the -80*C freezer. No freezing or thawing.
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