I'm working on extracellular xylanase and pectinase enzyme which was produce by fungsi via SSF.
In the enzyme assay for xylanase and pectinase I use pectin and xylan solution as substrate in the assay.
Is it ok for me to store the stock pectin and xylan substrate solution in 4 degree celsius for 3-4 weeks? Or it is better to prepare both solution fresh prior to enzyme assay?
I think that it should be better to use freshly prepared solution! Storage under refrigeration conditions not more than 1 week. For longer storage will be properly stored in a freezer.
I propose you, an study about stability of your solutions vs. time, during a monts. Only this way, you can know the stability.
Regards
Storage at 4° should be OK, better than freezing anyway, which will destabilize the solutions. And as pectin takes 24h to properly dissolve, I would not advise "freshly prepared". Just remember to add some merthiolate or sodium azide (100 mg/L) for microbial stability.
Dear Nizar. without underestimating the views of other distinguished researchers, based on their interesting laboratory experiments, suggest, as good practice, design an experiment to study the stability of the solutions. The reagents used are not always of the same quality, nor solvents, and neither are the working conditions or the experimentalist has the same ability ....., among other important aspects.
This methodological relativistic behavior is not surprising.
Rather, part of the resources of researchers to break paradigms and to encourage scientific research, which is far from dogmatism. It is not simple verificationism this proposal.
Greeting
Dear Nizzal,
in our lab we routinely use such substrates for enzyme assays. We add 0.02 % sodium azide (NaN3) to prevent contamination and store the substrates at 4 °C. Under these conditions, the substrates are stable for serveral weeks or months.
Dear Nizzal:
Undoubtedly, the experience of other researchers help to better some methods. I do special mention in not to follow exactly the methodological proposals of bibliography. This means, make effort in develop nobel methods. With creativity and critical sense, it is possible to reach this target. This is science, a permanent process of changes.
Regards
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