Nizzal Syafiq

11 Questions 12 Answers 0 Followers

Questions related from Nizzal Syafiq

How to improve the growth of Aspergillus versicolor fungus on PDA agar?

From my laboratory experiment, A. versicolor is able to grow on PDA agar but the growth is really slow compared to A. flavus and A. niger. It takes 2 weeks to make the A. versicolor cover 2/3 of...

25 November 2016 1,967 8 View

Do we need to plot zero absorbance for zero concentration in standard curve graph?

I'm currently doing glucose standard curve graph. Do I need to plot zero absorbance for zero concentration in the standard curve graph or just start with the first concentration that I used?

28 March 2016 5,741 7 View

Y-intercept is negative value for Xylose Standard Curve by DNS Method, is it normal?

I have managed to construct xylose standard curve by DNS method with R square value of 0.9985. With the equation y = 2.211x - 0.1607. Does this standard curve accepted to be use as refrecne if the...

18 March 2016 9,707 4 View

What is the best way to store pectin and xylan substrate solution?

I'm working on extracellular xylanase and pectinase enzyme which was produce by fungsi via SSF. In the enzyme assay for xylanase and pectinase I use pectin and xylan solution as substrate in the...

08 January 2016 4,845 6 View

What is the most accepted formula for enzyme activity calculation?

Does this formula accepted? Or they are other formula that are more widely used and accepted? Units (U/ml)= (microgram of glucose released) X (total assay volume) X dilution factor) / divided...

19 March 2015 4,696 15 View

Is it better to use rochelle salt before or after the addition of the DNS reagent?

In case of using crude enzyme samples, which we knew the properties of the crude enzyme is not pure and heterogenous. Is it better to use the DNS (with rochelle salt before) or use the DNS reagent...

09 March 2015 9,852 4 View

Do I have to do enzyme blank or buffer blank?

My research focus on the production of xylanase, and pectinase in SSF condition. I wanted to do enzyme assay of the crude enzyme extracted using DNS method. My question is, for each sample do I...

09 March 2015 3,966 8 View

Would it be okay if I use sodium acetate buffer to extract protease enzyme from fermented solid substrate (SSF)?

I'm currently conducting research on the production of xylanase, pectinase and protease via solid state fermentation. The crude enzyme I harvested from each set of SSF is my primary source of each...

06 March 2015 5,008 6 View

What is the best way to store crude enzyme extracted from solid state fermentation?

Currently I'm doing research on production of hydrolytic enzymes via SFF. Does anyone know the best way to store crude enzyme while maintaining its freshness in term of enzyme activity. And how...

03 March 2015 3,643 9 View

Which is between between spore suspension and plug inoculation methods?

I'm currently working on production of industrial enzyme via SSF. There is an ongoing argument on fungal inoculation either using plug inoculation or spore suspension inoculation methods. So my...

25 November 2014 2,637 4 View

How do we prevent condensation from happening on the inner top of a petri dish?

Currently, I am culturing fungus in petri dishes. Frequently, condensation of water occurred on the inner top of the petri dish. Although the water droplets didn't fall down it still blocks the...

14 November 2014 385 10 View