16 Questions 16 Answers 0 Followers
Questions related from Nizzal Syafiq
From my laboratory experiment, A. versicolor is able to grow on PDA agar but the growth is really slow compared to A. flavus and A. niger. It takes 2 weeks to make the A. versicolor cover 2/3 of...
25 November 2016 2,057 8 View
As we know DNS is an internationally acceptable method for xylanase enyzme activity assay. It has been use as a method to calculate the enzyme activity of xylanase by measuring the reducing sugar...
26 May 2016 1,419 2 View
I wanted to study the effect of 1 % nitrogen source on the enzyme production via Solid State Fermentation. Which method is correct? A). The 1% nitrogen source is determine based on the volume of...
22 April 2016 4,836 11 View
I'm currently doing glucose standard curve graph. Do I need to plot zero absorbance for zero concentration in the standard curve graph or just start with the first concentration that I used?
28 March 2016 5,809 7 View
I have managed to construct xylose standard curve by DNS method with R square value of 0.9985. With the equation y = 2.211x - 0.1607. Does this standard curve accepted to be use as refrecne if the...
18 March 2016 9,783 4 View
I'm working on extracellular xylanase and pectinase enzyme which was produce by fungsi via SSF. In the enzyme assay for xylanase and pectinase I use pectin and xylan solution as substrate in the...
08 January 2016 4,908 6 View
Does this formula accepted? Or they are other formula that are more widely used and accepted? Units (U/ml)= (microgram of glucose released) X (total assay volume) X dilution factor) / divided...
19 March 2015 4,775 15 View
Partial purification means the enzyme is partially purified, so what happen to other partial of the crude enzyme sample being subjected to this partial purification?
13 March 2015 9,068 4 View
In case of using crude enzyme samples, which we knew the properties of the crude enzyme is not pure and heterogenous. Is it better to use the DNS (with rochelle salt before) or use the DNS reagent...
09 March 2015 9,927 4 View
My research focus on the production of xylanase, and pectinase in SSF condition. I wanted to do enzyme assay of the crude enzyme extracted using DNS method. My question is, for each sample do I...
09 March 2015 4,022 8 View
I'm currently conducting research on the production of xylanase, pectinase and protease via solid state fermentation. The crude enzyme I harvested from each set of SSF is my primary source of each...
06 March 2015 5,067 6 View
Currently I'm working on the extraction of pectin from banana peel to make it as pectin powder. Does anyone have experience on this matter?
05 March 2015 5,510 3 View
Currently I'm doing research on production of hydrolytic enzymes via SFF. Does anyone know the best way to store crude enzyme while maintaining its freshness in term of enzyme activity. And how...
03 March 2015 3,716 9 View
I have a confusion regarding when do we have to prepare glucose standard graph as a reference for DNS assay of enzyme. Can we prepare it separately or we have to conduct both at the same time?
25 February 2015 8,911 9 View
I'm currently working on production of industrial enzyme via SSF. There is an ongoing argument on fungal inoculation either using plug inoculation or spore suspension inoculation methods. So my...
25 November 2014 2,686 4 View
Currently, I am culturing fungus in petri dishes. Frequently, condensation of water occurred on the inner top of the petri dish. Although the water droplets didn't fall down it still blocks the...
14 November 2014 428 10 View
