I want to test the cytotoxicity of some hydrogels on these cell types.
Dear Kirk Louie, it may be, these references will be helpful:
https://www.millipore.com/userguides/tech1/mcproto034
http://www.piercenet.com/product/mouse-embryonic-fibroblast-isolation-kit
http://www.piercenet.com/instructions/2162551.pdf
I am writing an article for my blog regarding orchid viruses aimed at home gardeners. It will include details of home testing kits their use and accuracy. I also want to include details about...
26 June 2024 7,422 1 View
I am studying polyethylene heat absorbing systems. For example, modeling heat exchanges in a solar air heater and temperatures for melting polyethylene.
01 June 2024 9,379 7 View
Hello, I want to get confirmation as to the correct way to specify a uniform EXTERNAL_PRESSURE in a non-orthogonal unit cell using the academic CASTEP code (i.e. not using the Materials studio...
29 May 2024 2,878 2 View
My research group would like to prepare some simulated intestinal fluid (SIF) with and without micelles, but we are not biochemists. Most methods we are finding (and commercial products) seem to...
23 May 2023 3,493 2 View
I'm having problems getting PloS One to accept a data availability statement for a paper currently in submission. This journal permits accession numbers for genetic sequences to be submitted...
30 May 2022 9,286 2 View
For comparison purposes, I wanted to get a list of all the currently-available experimental techniques that can be used to probe physical systems that have all the following characteristics: 1....
16 March 2022 3,707 1 View
What are the other ways in which I can do mixed methods? I'm just a beginner in the research arena. Maybe, you could provide also some resources here which can help me. Thank you!
22 December 2021 1,261 7 View
Hi, I am looking to estimate a willingness to pay for a choice model, but for the given alternatives compared to a base rather than for the product attributes variables. I have run a mixed logit...
22 August 2021 399 4 View
I am doing a research paper for my pgce and I need to find a case study on current modern families in South Africa. Then a case study of past families in South Africa back dating to about 20...
14 July 2020 3,163 0 View
Qiagen column extraction for rt-rt-PCR. Occasional contamination (from glove?) to next samples. Occasional contamination to random samples within batch (centrifuge/vortex?).
19 May 2020 6,614 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View