My research group would like to prepare some simulated intestinal fluid (SIF) with and without micelles, but we are not biochemists. Most methods we are finding (and commercial products) seem to mix sodium taurocholate with lecithin to prepare SIF. However, we had purchased some "bile salts" (50/50 mix of cholate and deoxycholate) which are less expensive than the taurocholate and were wondering if this is also considered appropriate for SIF although we have only found one reference so far that uses those with lecithin. Would anyone know if there is a chemical reason for using taurocholate instead of cholate/deoxycholate? Would it be considered "acceptable" to use the cholate/deoxycholate mixture, or would a reviewer expect taurocholate needs to be used?
Stacey Louie
It has been shown that taurocholate is a powerful stimulator of the release of three hormonal peptides in the wall of the ileum, i.e. peptide YY (PYY), glucagon-like peptide-1 (GLP-1), and neurotensin (NT)
Dumoulin V., Moro F., Barcelo A. et al. Peptide YY, glucagonlike peptide-1, and neurotensin responses to luminal factors in the isolated vascularly perfused rat ileum // Endocrinology. - 1998. - Sep. – Vol. 139, No. 9. - P. 3780-3786.
Dear Stacey,
I hope this may help you:
Sodium taurocholate was chosen as a representative bile salt because cholic acid is one of the more prevalent bile salts in human bile. Furthermore, since the taurine conjugate has a very low pKa, there is little likelihood of its precipitation or a change in the micellar size with minor variations in pH value within the typical range found in the proximal small intestine (pH 4–7).
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