Qiagen column extraction for rt-rt-PCR.
Occasional contamination (from glove?) to next samples.
Occasional contamination to random samples within batch (centrifuge/vortex?).
I am writing an article for my blog regarding orchid viruses aimed at home gardeners. It will include details of home testing kits their use and accuracy. I also want to include details about...
26 June 2024 7,422 1 View
I am studying polyethylene heat absorbing systems. For example, modeling heat exchanges in a solar air heater and temperatures for melting polyethylene.
01 June 2024 9,379 7 View
Hello, I want to get confirmation as to the correct way to specify a uniform EXTERNAL_PRESSURE in a non-orthogonal unit cell using the academic CASTEP code (i.e. not using the Materials studio...
29 May 2024 2,878 2 View
I'm having problems getting PloS One to accept a data availability statement for a paper currently in submission. This journal permits accession numbers for genetic sequences to be submitted...
30 May 2022 9,286 2 View
For comparison purposes, I wanted to get a list of all the currently-available experimental techniques that can be used to probe physical systems that have all the following characteristics: 1....
16 March 2022 3,707 1 View
I have simulated in silico how to clone a large protein (1900bp) Please, any suggestions on what measures to consider before doing wet lab to clone and express this protein? Thank you for your...
28 October 2021 2,817 5 View
I am doing a research paper for my pgce and I need to find a case study on current modern families in South Africa. Then a case study of past families in South Africa back dating to about 20...
14 July 2020 3,163 0 View
I am trying to run a TaqMan assay where my housekeeping probe is labelled with VIC. I do not have access to a qPCR machine with VIC specifically defined, but HEX is recommended as a substitute. Is...
18 August 2019 9,401 4 View
Hi I am trying to analyse the effectiveness of strength training program on time trial performance for different sports, running, cycling swimming etc. As the distances, mode and unit of...
09 February 2019 725 5 View
1. Transfection: I need a double knockdown protocol because breeding is too slow. 2. Lipofectamine: I used RNAiMAX. 3. Cell density: I used 6-well plates, 160K cells per plate (the true macrophage...
02 December 2018 1,655 3 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View