The neurospheres are washed off during the fixation and washing steps. I need a better way to let them adhere on the surface and then perform the steps.
Another option is to use a cytospin if you have access to one. You can use this to directly spin your floating neurospheres onto poly-l-lysine slides, fix the spheres and then proceed as normal with staining. Very easy but depends on whether you have access to a cytospin machine.
Another option is to use a cytospin if you have access to one. You can use this to directly spin your floating neurospheres onto poly-l-lysine slides, fix the spheres and then proceed as normal with staining. Very easy but depends on whether you have access to a cytospin machine.
Hi Sean, Thanks. I have tried with laminin but I lost the spheres during the fixing or washing steps. In regard to second option, Would centrifugation affect the sphere morphology?
Hi Fiona, thanks but we do not have cytospin in lab.
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