I was trying to label synapses in brain slices using a Anti-PSD 95 primary antibody (Host-Rabbit) and a Anti-Rabbit Alexa 488 secondary antibody. I was wondering how long I should incubate the antibodies for 20 and 60 micron slices?
Also, is ethanol dehydration necessary after fixation or I can directly incubate the tissue with antibody after fixing and blocking?
Thanks