04 January 2019 1 3K Report

I was trying to label synapses in brain slices using a Anti-PSD 95 primary antibody (Host-Rabbit) and a Anti-Rabbit Alexa 488 secondary antibody. I was wondering how long I should incubate the antibodies for 20 and 60 micron slices?

Also, is ethanol dehydration necessary after fixation or I can directly incubate the tissue with antibody after fixing and blocking?

Thanks

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