Surya Aryal

5 Questions 8 Answers 0 Followers

Questions related from Surya Aryal

Has anyone used filament tracer tool to analyze brain vasculature?

I am trying to measure morphological parameters in 3-dimensional blood vasculature data. I have seen people using filament tracer in imaris to analyze neurons and synapses. I was wondering whether...

08 November 2021 665 0 View

Has anyone used any astrocyte specific AAV-PHP?

I am looking for an astrocyte-specific AAV-PHP to perform experiments in vivo. I know people have used AAV-PHP for neurons and other cells but I couldn't find any specific AAV-PHP which only...

26 February 2020 1,870 3 View

Does paraformaldehyde fixing change the stiffness of the cell?

I was planning to perform young's moduli measurment of mammalian cells with atomic force microscopy (AFM). The live cells migrate when you try to perform AFM. I am thinking to fix the cells so...

07 August 2019 3,346 3 View

How long cells can survive in L-15 media at room temperature without CO2?

I was trying to perform imaging with mixed glial cells in L-15 media. The microscope had some issue so I couldn't image them on time. I was wondering whether I can still image the cells in L-15...

16 January 2019 9,557 0 View

What is the best way to label synapses (post synaptic density) in brain slices with Anti-PSD 95 antibody?

I was trying to label synapses in brain slices using a Anti-PSD 95 primary antibody (Host-Rabbit) and a Anti-Rabbit Alexa 488 secondary antibody. I was wondering how long I should incubate the...

04 January 2019 3,283 1 View