What is the best way to isolate high quality mitochondria from cells (osteosarcoma) to perform a Blue native PAGE for studying super complexes?

24 January 2014 0 4K Report

I am trying to study the respiratory supercomplexes in osteosarcoma cells by BN-PAGE

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Science method

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Is it possible to use the Fused Deposition Modeling (FDM) to additively manufacture interconnected porous structure generation of >100-200 micrometer?

Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...

09 August 2024 7,892 0 View

How can we deal immediately with a person having anxiety attacks?

there can be some triggering situations, how do you handle them?

18 July 2024 7,076 3 View

What is the degree of crystallinity of a crosslinked polyethylene (PEX-A) pipe? Is there some kind of index? If yes, where can I find them?

I need the crystallinity percentage value of crosslinked polyethylene (PEX-A). I know that they have more than 70% crosslinking. Is there any way to calculate the crystallinity percentage from...

22 April 2024 3,007 0 View

How to check dipole-dipole interaction strength between two molecules when H bonding may not be the stabilising factor in a dimer molecule?

I have optimised the geometry of the dimer of a molecule using pcm solvent model and B3LYP functional with 6311++G(d,p) basis set in Gaussian 09 software. There is possibility of C-H...O H bonding...

01 April 2024 1,820 1 View

How to dissolve Pyrazinamide to administer in mice?

Hi, I intend to give mice 150 mg/kg of pyrazinamide. I need 3.7mg per mice and 100ul needs to be administered. The drug only dissolves in DMSO and the final amount of the DMSO in water exceeds...

27 September 2023 6,416 0 View

Without measuring lifetime, how to distinguish between fluorescence and delayed fluorescence?

The PL int will be higher in the case of Fl, but both are supposed to overlap. Then how do know if the system has delayed Fl emission?

05 September 2023 3,068 4 View

How a flame within deep tube well water in remote village can be explained as well as eliminated ?

Image Ref : The Telegraph Online ( 16.06.18 )/ 19.07.2023.

18 July 2023 397 8 View

How to calculate ionic radii of cation and anion for a triclinic cell?

I have crystal structure of a compound (a salt) which is a triclinic cell. Now I want to determine the radius of cation and anion. How can I do it please explain. Many thanks in advance. Looking...

23 May 2023 1,634 3 View

How can one determine equilibrium constant of a reaction by 1H NMR spectroscopy?

Suppose a chemical reaction like: AH + B = A + BH, where AH is a paramagnetic metal complex with acidic hydrogen and B is a base. I want to determine the equilibrium constant of this reaction in...

18 April 2023 2,831 6 View

What is the difference between PBS and Sodium Phosphate?

Sodium Phosphate manufacture uses sodium dihydrogen phosphate and disodium hydrogen phosphate. PBS manufacture uses pottasium ohosphate monobasic, sodium phosphate dibasic and sodium chloride....

03 April 2023 1,039 2 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View