What is the best way to isolate high quality mitochondria from cells (osteosarcoma) to perform a Blue native PAGE for studying super complexes?

24 January 2014 0 4K Report

I am trying to study the respiratory supercomplexes in osteosarcoma cells by BN-PAGE

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Science method

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How to implement boundary conditions in Abaqus contact problems?

Hello I am trying to simulate crawling motion of a bilayer beam using Abaqus. Kind of similar to this: https://youtu.be/os-Z3ucoFpA I am using multiphysics enabled (diffusion, deformation,...

20 February 2021 2,916 1 View

Do we see connection between climate change and the transmission of coronavirus?

How likely are we to see the infectious disease spread as a result of climate change? How are the communities most at-risk, and how and why both COVID-19 and climate change harms them? Why is it...

09 February 2021 1,459 28 View

I get tumor tissue from the patients after the surgery. How to isolate the top layer of cells from a tumor?

I work on gastric cancer. I want to separate the top layer of tumor cells from the lower layers.

25 January 2021 6,663 1 View

How to decolonize climate change research?

Who has the right to define what is climate change research should be? Who should define the climate change research topic, research questions, and research design? Who should own the climate...

29 November 2020 1,077 12 View

What are the outstanding issues in laser glass?

Laser glass is essentially Nd-doped phosphate glass. It is used as the gain medium in high power lasers. The questions are: (1) Why phosphate glasses are important? (2) Can they be replaced by...

24 November 2020 1,286 6 View

Is the future of "Science" or "the Scientific Implications of Climate Change" connected with the US election?

Donald Trump and his challenger Joe Biden have presented vastly different visions for handling crucial issues, from the coronavirus pandemic to climate change. This results in how the election...

29 October 2020 8,810 6 View

Why after importing .shp files for microCFD 2D simulation the dimensions of the figure appears to be changed?

I am currently trying to do a microCFD simulation of a figure that has got some definite dimensions but after importing in the microCFD and then clicking on the modify shape option from the edit...

10 October 2020 3,272 1 View

In FullProf XRD Fitting, which FWHM parameters(u,v,w) should be negative?

Generally v is negative initially but what it should be after fitting. what does a negative sign or positive sign of u,v,w mean ?

02 August 2020 7,907 5 View

How to find properties of a network plot?

Hi, I constructed a network with igraph showing the connections between bacterial groups in three different systems (suppose A, B and C). Each node represented an OTU/ASV and edge represented...

21 June 2020 7,637 1 View

Are you writing your self-resilience stories from the Covid-19 disaster?

How are you taking positive steps against the Covid-19 disaster? How are you protecting your community? What do you want to suggest?

23 March 2020 7,820 38 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View