Hi Everyone, I am working with head and neck cancer FFPE samples. I am going to validate some miRNA by quantitative PCR. What is the best way to find best endogenous control for miRNA? Thank you in advance for your answer and suggestions. Parwez
Try to run a standard curve of your interested gene and internal control. Both should come parallel. U6 (4.8S) nuclear RNA, is a good internal control for miRNA.