9 Questions 17 Answers 0 Followers
Questions related from Parwez Ahmad
Dear Researchers, Does anyone has experience in measuring vortex shaker rpm speed into orbital flow shear stress in dyne/cm2? I am doing some experiment on endothelial cells and need to establish...
09 September 2021 7,673 0 View
Dear Researchers, Can anyone share the experience with the expansion of mouse NK cells after isolation? I can see there are kits for the isolation of NK cells from mice but I can't find any media...
11 May 2021 847 3 View
Dear Researchers, I am working with miRNAs from the tick's salivary gland, midgut, and saliva to study the involvement of these miRNAs in host-vector interaction for successful blood feeding. Do...
01 February 2021 4,478 2 View
Dear researchers, I have ordered customized primers for ticks candidate miRNAs as these miRNAs are not available in any company. I have used a miRNA-specific MystiCq cDNA synthesis kit for cDNA...
15 October 2020 5,740 7 View
Dear researchers, I am facing some issues with Sybre green qPCR results. I have isolated total RNA from different organs of ticks using the Trizol RNA isolation kit. After cDNA preparation with a...
15 September 2020 5,210 5 View
Hello everyone, I am working with qPCR and relative gene expression study of some candidate miRNAs. How many methods are there which can be used to normalize the PCR data? I know most famous is...
13 March 2018 9,718 4 View
Hi there, I am working with TaqMan Advanced miRNA assay as per protocol but I am not getting significant Ct values. My samples are total RNA enriched with small RNA from FFPE samples. I am using...
31 January 2018 2,561 7 View
Hi Everyone, I am working with head and neck cancer FFPE samples. I am going to validate some miRNA by quantitative PCR. What is the best way to find best endogenous control for miRNA? Thank you...
02 February 2017 4,560 2 View
Hello Everyone, Please share your experience. Thank you.
17 October 2016 9,325 4 View